Pharmacological assessment of Ru(II) complex with GidA protein- A novel topoisomerase II inhibitor towards cancer therapeutics

被引：0

作者：

Infanta, Antony K. Teresa S. ^{[1
,4
]}

Durairaju, Nisshanthini ^{[1
]}

Raja, Senthil ^{[2
]}

Murugesan, Thandeeswaran ^{[1
]}

Dhanapal, Anand Raj ^{[3
]}

Natarajan, Karupannan ^{[2
]}

Balakrishnan, Ajithkumar ^{[5
]}

Vedagiri, Hemamalini ^{[5
]}

Muthusamy, Palaniswamy ^{[6
]}

Jayaraman, Angayarkanni ^{[1
]}

机构：

[1] Bharathiar Univ, Dept Microbial Biotechnol, Canc Therapeut Lab, Coimbatore, Tamil Nadu, India

[2] Bharathiar Univ, Dept Chem, Coimbatore, Tamil Nadu, India

[3] Karpagam Acad Higher Educ, Dept Biotechnol, Coimbatore, Tamil Nadu, India

[4] New Prince Shri Bhavani Arts & Sci Coll, Dept Biotechnol, Chennai, Tamil Nadu, India

[5] Bharathiar Univ, Dept Bioinformat, Mol Genom Lab, Coimbatore, Tamil Nadu, India

[6] Karpagam Acad Higher Educ, Dept Microbiol, Coimbatore, Tamil Nadu, India

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 2023年 / 41卷 / 09期

关键词：

Ruthenium(II) complexes; GidA protein; UV-absorption spectroscopy; fluorescence quenching; CD spectroscopy; SERUM-ALBUMIN; DNA TOPOISOMERASE; RUTHENIUM COMPLEXES; ADDUCT FORMATION; BINDING; COORDINATION; REDUCTION; MODE;

D O I：

10.1080/07391102.2022.2064332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions of ruthenium(II) complex with Glucose inhibited division protein A (GidA protein) was studied through various spectroscopic techniques with the ultimate goal of preparing adducts with good selectivity for cancer cells. In all the cases, formation of a tight metal-protein conjugate was observed. The influence of pH, reducing agents and chelators on the formation of adduct was analysed by UV- visible spectroscopy. While there was no effect on the addition of sodium ascorbate, some alterations on some selected bands were seen on the UV-visible spectra on the addition of EDTA. The adduct was stable in the pH range of 5-8. Addition of ruthenium(II) complex effectively quenched the intrinsic fluorescence of GidA and it occurred through static quenching. The effect of ruthenium(II) complex on the conformation of GidA has been examined by analyzing CD spectrum. Though, there was some conformational changes observed in the presence of ruthenium(II) complex, alpha- helix in the secondary structure of GidA retained its identity. Molecular docking of ruthenium(II) complex with GidA also indicated that GidA docks through hydrophobic interaction. The stable semisynthetic complex (ruthenium(II) complex with GidA) was checked for topoisomerase II inhibition. Relaxation and decatenation assay proved topoisomerase II inhibition of semisynthetic complex. Communicated by Ramaswamy H. Sarma

引用

页码：4143 / 4153

页数：11