Optogenetic-mediated induction and monitoring of a-synuclein aggregation in cellular models of Parkinson's disease

被引：0
|
作者：
Teixeira, Maxime [1 ,2 ]
Sheta, Razan [1 ,2 ]
Idi, Walid [1 ,2 ]
Oueslati, Abid [1 ,2 ]
机构：
[1] CHU Quebec Res Ctr, Axe Neurosci, Quebec City, PQ, Canada
[2] Univ Laval, Fac Med, Dept Mol Med, Quebec City, PQ, Canada
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
加拿大健康研究院;
关键词：
TRANSGENE EXPRESSION;
D O I：
10.1016/j.xpro.2023.102738
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Studying Parkinson's disease (PD) is complex due to a lack of cellular models mimicking key aspects of protein pathology. Here, we present a protocol for inducing and monitoring a-synuclein aggregation in living cells using optogenetics. We describe steps for plasmid transduction, biochemical validation, immunocytochemistry, and live-cell confocal imaging. These induced aggregates fulfill the cardinal features of authentic protein inclusions observed in PD -diseased brains and offer a tool to study the role of protein aggregation in neurodegeneration. For complete details on the use and execution of this protocol, please refer to Be ' rard et al.1
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页数：29
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