Optogenetic induction of alpha-synuclein aggregation in human dopaminergic neurons to model Parkinson's disease pathology

被引：3
|
作者：
Ra, Eun A. [1 ,2 ]
Kim, Min Seong [1 ,2 ]
Lee, Gabsang [1 ,2 ,3 ]
机构：
[1] Johns Hopkins Univ, Inst Cell Engn, Sch Med, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA
[3] Johns Hopkins Univ, Sch Med, Solomon Snyder Dept Neurosci, Baltimore, MD 21205 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
美国国家卫生研究院;
关键词：
Cell Differentiation; Organoids; Stem Cells;
D O I：
10.1016/j.xpro.2023.102609
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Alpha-synuclein (a-syn) aggregation is a principal factor in Parkinson's disease (PD) onset. Here, we present a protocol for optogenetic induction of a-syn aggregation in human midbrain dopaminergic (mDA) neurons, facilitating a detailed PD pathology study. We describe steps for nucleofection of the opto-a-syn construct, single colony selection and validation, alongside mDA neuron differentiation and rapid induction of toxic a-syn aggregates via blue light. This establishes a potent human induced pluripotent-stem-cell-based platform for PD drug testing and validation.For complete details on the use and execution of this protocol, please refer to Kim et al. (2023).1
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页数：24
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