Protocol Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

被引：3

作者：

Giacomoni, Jessica ^{[1
]}

Habekost, Mette ^{[1
,2
]}

Cepeda-Prado, Efrain ^{[3
]}

Mattsson, Bengt ^{[1
]}

Ottosson, Daniella Rylander ^{[3
]}

Parmar, Malin ^{[1
]}

Kajtez, Janko ^{[1
]}

机构：

[1] Lund Univ, Wallenberg Neurosci Ctr, Lund Stem Cell Ctr, Dept Expt Med Sci,Dev & Regenerat Neurobiol, Lund, Sweden

[2] Univ Copenhagen, Fac Hlth & Med Sci, Ctr Neurosci, Copenhagen, Denmark

[3] Lund Univ, Wallenberg Neurosci Ctr, Lund Stem Cell Ctr, Dept Expt Med Sci,Regenerat Neurophysiol, Lund, Sweden

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

瑞典研究理事会; 欧盟地平线“2020”;

关键词：

Cell Biology; Cell culture; Microscopy; Model Organisms; Neuroscience;

D O I：

10.1016/j.xpro.2022.102041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also pro-vide instructions for culturing of rat brain slices free floating on permeable cul-ture inserts, maintained in regular CO2 incubators, and handled only at media change.

引用

页数：19

共 50 条

[11] Optimized magnetic resonance diffusion protocol for ex-vivo whole human brain imaging with a clinical scanner
Scherrer, Benoit
Afacan, Onur
Stamm, Aymeric
Singh, Jolene
Warfield, Simon K.
MEDICAL IMAGING 2015: PHYSICS OF MEDICAL IMAGING, 2015, 9412
[12] A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana
Musielak, Thomas J.
Slane, Daniel
Liebig, Christian
Bayer, Martin
PLOS ONE, 2016, 11 (08):
[13] Analysis of Propagation of Slow Rhythmic Activity Induced in Ex Vivo Rat Brain Slices
Balogh, Veronika
Szadeczky-Kardoss, Katalin
Varro, Petra
Vilagi, Ildiko
Borbely, Sandor
BRAIN CONNECTIVITY, 2019, 9 (08) : 649 - 660
[14] EX VIVO IMAGING OF ULTRASOUND-STIMULATED METABOLIC ACTIVITY IN RAT PANCREATIC SLICES
Chen, Andrew W.
Jeremic, Aleksandar
Zderic, Vesna
ULTRASOUND IN MEDICINE AND BIOLOGY, 2021, 47 (03): : 666 - 678
[15] A reliable indirect cell-labelling protocol for optical imaging allows ex vivo visualisation of mesenchymal stem cells after transplantation in rodent brain
Diana, V.
Libani, I. V.
Armentero, M. -T.
Blandini, F.
Lucignani, G.
Silani, V.
Cova, L.
Ottobrini, L.
ARCHIVES ITALIENNES DE BIOLOGIE, 2013, 151 (03): : 114 - 125
[16] Protocol to study presynaptic protein synthesis in ex vivo mouse hippocampal slices using HaloTag technology
Kharod, Shivani C.
Monday, Hannah R.
Yoon, Young J.
Castillo, Pablo E.
STAR PROTOCOLS, 2023, 4 (01):
[17] Adaptive light sheet microscopy for in vivo imaging of fluorescently labeled specimens
Mylonakis, Mikis
Zacharopoulos, Athanasios
Marakis, Evangelos
Papazoglou, Dimitrios G.
Zacharakis, Giannis
ADVANCES IN MICROSCOPIC IMAGING III, 2021, 11922
[18] Viscous optical clearing agent for in vivo optical imaging
Deng, Zijian
Jing, Lijia
Wu, Ning
Lv, Pengyu
Jiang, Xiaoyun
Ren, Qiushi
Li, Changhui
JOURNAL OF BIOMEDICAL OPTICS, 2014, 19 (07)
[19] ALTERATIONS IN AUTOFLUORESCENCE SIGNAL FROM RAT SKIN EX VIVO UNDER OPTICAL IMMERSION CLEARING
Migacheva, E. V.
Pravdin, A. B.
Tuchin, V. V.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, 2010, 3 (03) : 147 - 152
[20] Optical clearing of skin tissue ex vivo with polyethylene glycol
D. K. Tuchina
V. D. Genin
A. N. Bashkatov
E. A. Genina
V. V. Tuchin
Optics and Spectroscopy, 2016, 120 : 28 - 37

← 1 2 3 4 5 →