Protocol Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

被引：3

作者：

Giacomoni, Jessica ^{[1
]}

Habekost, Mette ^{[1
,2
]}

Cepeda-Prado, Efrain ^{[3
]}

Mattsson, Bengt ^{[1
]}

Ottosson, Daniella Rylander ^{[3
]}

Parmar, Malin ^{[1
]}

Kajtez, Janko ^{[1
]}

机构：

[1] Lund Univ, Wallenberg Neurosci Ctr, Lund Stem Cell Ctr, Dept Expt Med Sci,Dev & Regenerat Neurobiol, Lund, Sweden

[2] Univ Copenhagen, Fac Hlth & Med Sci, Ctr Neurosci, Copenhagen, Denmark

[3] Lund Univ, Wallenberg Neurosci Ctr, Lund Stem Cell Ctr, Dept Expt Med Sci,Regenerat Neurophysiol, Lund, Sweden

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

瑞典研究理事会; 欧盟地平线“2020”;

关键词：

Cell Biology; Cell culture; Microscopy; Model Organisms; Neuroscience;

D O I：

10.1016/j.xpro.2022.102041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also pro-vide instructions for culturing of rat brain slices free floating on permeable cul-ture inserts, maintained in regular CO2 incubators, and handled only at media change.

引用

页数：19

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