Effect of Panax ginseng and Fructus Mume on Intestinal Barrier and Gut Microbiota in Rats with Diarrhea

被引:10
|
作者
Zhao, Mengjie [1 ]
Zhao, Qiong [1 ,6 ]
Guan, Zhiwei [2 ]
Liu, Qianwei [3 ,4 ]
Zhou, Hongyun [2 ]
Huang, Qinwan [5 ]
Huo, Bixiu [1 ]
机构
[1] Hosp Chengdu Univ Tradit Chinese Med, Dept Pediat, Chengdu, Peoples R China
[2] Henan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Pediat, Zhengzhou, Peoples R China
[3] Beijing Univ Chinese Med, Sch Clin Med, Beijing, Peoples R China
[4] China Japan Friendship Hosp, Dept Dermatol & Venereol, Beijing, Peoples R China
[5] Chengdu Univ Tradit Chinese Med, Sch Pharm, Chengdu, Peoples R China
[6] Hosp Chengdu Univ Tradit Chinese Med, Dept Pediat, 1166 Liutai Ave, Chengdu 611137, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
diarrhea; Fructus Mume; gut microbiota; intestinal barrier; Panax ginseng; PI3K/Akt/NF-kBsignaling pathway; PERSISTENT DIARRHEA; BIOLOGY;
D O I
10.1089/jmf.2022.K.0069
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Panax ginseng and Fructus mume (Renshen Wumei in Chinese, RW) are natural medicines with high nutritional and pharmacological value. They have been widely used together in China to treat gastrointestinal diseases, especially persistent diarrhea, but the potential mechanisms remain elusive. In this study, a diarrhea model was established in rats using a 30% aqueous extract of senna. The therapeutic effects of RW were evaluated by recording the prevalence of loose stools, the diarrhea index, and histopathological changes in colon tissue. The levels of mucins, tight junction (TJ) proteins, inflammatory cytokines, and phosphoinositide 3-kinase/Akt/nuclear factor-kappa B (PI3K/Akt/NF-kappa B) signaling pathway proteins were measured. Metagenomic sequencing was used to analyze the gut microbiota. Treatment with RW alleviated injury to the intestinal barrier in rats with diarrhea and also upregulated levels of Muc2 and TJ proteins, such as occludin, zonula occludens-1, and claudin-1. Administration of RW regulated the structure of the gut microbiota in diarrheal rats. Furthermore, RW suppressed levels of interleukin (IL), tumor necrosis factor (TNF)-alpha, IL-1, PI3K, Akt, and p-NF-kappa B p65 and also increased IL-4 levels. Our study indicates that P. ginseng and Fructus mume help improve the symptoms of diarrhea, possibly by alleviating the intestinal barrier injury, regulating intestinal flora composition, and inhibiting the PI3K/Akt/NF-kappa B signaling pathway.
引用
收藏
页码:165 / 175
页数:11
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