Chromosome-level genome assembly of the flower thrips Frankliniella intonsa

被引:5
|
作者
Zhang, Zhijun [1 ]
Bao, Jiandong [1 ]
Chen, Qizhang [1 ,3 ]
He, Jianyun [1 ]
Li, Xiaowei [1 ]
Zhang, Jiahui [1 ,2 ]
Liu, Zhixing [1 ]
Wu, Yixuan [1 ]
Li, Xuesheng [3 ]
Wang, Yunsheng [2 ]
Lu, Yaobin [1 ]
机构
[1] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
[2] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha 410125, Peoples R China
[3] Guangxi Univ, Guangxi key Lab Agr Environm & Agr Prod Safety, Nanning 530004, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
THYSANOPTERA THRIPIDAE; TOOL; ANNOTATION; ACCURATE;
D O I
10.1038/s41597-023-02770-3
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis and evolution of F. intonsa. In this study, we used Oxford Nanopore Technology (ONT) long reads and High-through chromosome conformation capture (Hi-C) linked reads to construct a high-quality reference genome assembly of F. intonsa, with a total size of 225.5 Mb and a contig N50 of 3.37 Mb. By performing the Hi-C analysis, we anchored 91.68% of the contigs into 15 pseudochromosomes. Genomic annotation uncovered 17,581 protein-coding genes and identified 20.09% of the sequences as repeat elements. BUSCO analysis estimated over 98% of genome completeness. Our study is at the first time to report the chromosome-scale genome for the species of the genus Frankliniella. It provides a valuable genomic resource for further biological research and pest management of the thrips.
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页数:6
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