Structural and functional properties of collagen isolated from lumpfish and starfish using isoelectric precipitation vs salting out

被引：16
|
作者：
Vate, Naveen Kumar [1 ,2 ]
Strachowski, Przemyslaw [1 ]
Undeland, Ingrid [1 ]
Abdollahi, Mehdi [1 ]
机构：
[1] Chalmers Univ Technol, Dept Life Sci Food & Nutr Sci, S-41296 Gothenburg, Sweden
[2] Centurion Univ Technol & Management, Sch Fisheries, Dept Fish Proc Technol, Paralakhemundi 761211, Odisha, India
来源：
FOOD CHEMISTRY-X | 2023年 / 18卷
关键词：
Marine collagen; Lumpfish; Starfish; Isoelectric precipitation; Fibril formation; ACID-SOLUBLE COLLAGEN; PEPSIN-SOLUBILIZED COLLAGEN; BIOCHEMICAL-CHARACTERIZATION; SKIN COLLAGEN; EXTRACTION; TEMPERATURE; STABILITY; PROTEINS; ABILITY; SCALES;
D O I：
10.1016/j.fochx.2023.100646
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The possibility of replacing the very time and resource demanding salting out (SO) method with isoelectric precipitation (IP) during collagen extraction from common starfish and lumpfish was investigated. The effect of IP on yield, structural and functional properties of the collagens was therefore compared with SO. Application of IP resulted in a higher or similar collagen mass yield compared with SO from starfish and lumpfish, respectively. However, the purity of collagens recovered with IP was lower than those recovered with SO. Replacing SO with IP did not affect polypeptide pattern and tropohelical structural integrity of collagen from the two resources as revealed with SDS-PAGE and FTIR analysis. Thermal stability and fibril formation capacity of collagens recovered with IP were also well preserved. Overall, the results showed that the IP can be a promising resource smart alternative for the classic SO precipitation during collagen extraction from marine resources.
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收藏
页数：9
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