Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

被引：3

作者：

Labora, Amanda ^{[1
]}

Lee, Hailey ^{[1
]}

Chan, Charlotte ^{[1
]}

Tabornal, Erin

Le, Thuc ^{[2
]}

Rashid, Khalid ^{[2
]}

Abt, Evan ^{[2
]}

Yamao, Takanobu ^{[1
]}

Mandl, Hanna ^{[2
]}

Creech, Amanda ^{[2
]}

Premji, Alykhan ^{[1
]}

Li, Luyi ^{[1
]}

Link, Jason ^{[1
]}

Wu, Nanping ^{[1
]}

Radu, Caius ^{[2
]}

Donahue, Timothy ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Surg, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Ahmanson Imaging Translat Div, Los Angeles, CA 90095 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Cancer; Cell Biology; Health Sciences; Model Organisms;

D O I：

10.1016/j.xpro.2023.102163

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for inject-ing tumor cells. One technique is freehand, while the other technique is device -assisted using a 3D-printed prototype device. Finally, we describe tumor surveil-lance with bioluminescent imaging.

引用

页数：20

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