The Role of lncRNAs in Pig Muscle in Response to Cold Exposure

被引:1
|
作者
Zhang, Dongjie [1 ]
Wang, Liang [1 ]
Wang, Wentao [1 ]
Liu, Di [1 ]
机构
[1] Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China
基金
中国国家自然科学基金;
关键词
pig; skeletal muscle; low temperature; lncRNA; functional analysis; LOW-TEMPERATURE; THERMOGENESIS; INVOLVEMENT; ACCLIMATION;
D O I
10.3390/genes14101901
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cold exposure is an essential factor affecting breeding efforts in cold regions. Muscle, as an important tissue for homeothermic animals, can produce heat through shivering thermogenesis (ST) and non-shivering thermogenesis (NST) under cold exposure. Long non-coding RNAs (lncRNAs) play important roles in regulating gene expression. However, the regulatory mechanisms of lncRNAs and their role in the thermogenesis of pigs are unclear. We examined lncRNAs in the skeletal muscle of an indigenous pig breed, the Enshi black pig, when the pigs were exposed to acute or chronic cold. Three pigs were maintained inside a pig house (control group), three pigs were maintained outside the pig house for 55 d (chronic cold group), and three pigs were suddenly exposed to the conditions outside the pig house for 3 days (acute cold group). After the experiment, the longissimus dorsi of each pig were collected, and their lncRNA profiles were sequenced and analyzed. Each sample obtained nearly 12.56 Gb of clean data. A total of 11,605 non-coding RNAs were obtained, including 10,802 novel lncRNAs. The number of differentially expressed lncRNAs (DElncRNAs) was identified under acute cold (427) and cold acclimation (376), with 215 and 192 upregulated lncRNAs, respectively. However, only 113 lncRNAs were commonly upregulated by acute cold and cold acclimation. In addition, 65% of the target genes were trans-regulated by DElncRNAs. The target genes were enriched in signal transduction, immune system, cell growth and death pathways, and amino acid and carbohydrate metabolism. Compared to cold acclimation, acute cold stress-induced more DElncRNAs and response pathways. In conclusion, low temperatures altered the expression levels of lncRNAs and their target genes in muscle tissue. Some potential mechanisms were revealed, including ion migration and the metabolism of amino acids and carbohydrates.
引用
收藏
页数:15
相关论文
共 50 条
  • [21] Transcriptome sequencing reveals the differentially expressed lncRNAs and mRNAs in response to cold acclimation and cold stress in Pomacea canaliculata
    Xiao, Qi
    Lin, Youfu
    Li, Hong
    Chen, Yu
    Wei, Wei
    Li, Peng
    Chen, Lian
    BMC GENOMICS, 2022, 23 (01)
  • [22] Transcriptome sequencing reveals the differentially expressed lncRNAs and mRNAs in response to cold acclimation and cold stress in Pomacea canaliculata
    Qi Xiao
    Youfu Lin
    Hong Li
    Yu Chen
    Wei Wei
    Peng Li
    Lian Chen
    BMC Genomics, 23
  • [23] The role of lncRNAs and exosomal lncRNAs in cancer metastasis
    Zhang, Yutong
    Zhao, Lijuan
    Bi, Yaping
    Zhao, Jinyuan
    Gao, Chao
    Si, Xiaojie
    Dai, Honglin
    Asmamaw, Moges Dessale
    Zhang, Qiurong
    Chen, Wenchao
    Liu, Hongmin
    BIOMEDICINE & PHARMACOTHERAPY, 2023, 165
  • [24] Experimental Models for Cold Exposure of Muscle in vitro and in vivo
    Schjolberg, Tiril
    Asoawe, Lucia
    Krapf, Solveig
    Rustan, Arild
    Thoresen, G. Hege
    Haugen, Fred
    BIO-PROTOCOL, 2022, 12 (13):
  • [25] METABOLISM AND MUSCLE ACTIVITY OF ANESTHETIZED WARM AND COLD ACCLIMATED RATS ON EXPOSURE TO COLD
    HEROUX, O
    HART, JS
    DEPOCAS, F
    JOURNAL OF APPLIED PHYSIOLOGY, 1956, 9 (03) : 399 - 403
  • [26] Sex difference of arterial response to cold exposure
    Sato, Fujio
    Matsushita, Shonosuke
    Hyodo, Kazuyuki
    Enomoto, Yoshiharu
    Hiramatsu, Yuji
    Sakakibara, Yuzuru
    FASEB JOURNAL, 2007, 21 (06): : A1371 - A1371
  • [27] Physiological response of rodents to prolonged cold exposure
    Reges, C.
    Schaeffer, P. J.
    INTEGRATIVE AND COMPARATIVE BIOLOGY, 2023, 62 : S260 - S261
  • [28] lncRNAs in Stress Response
    Valadkhan, Saba
    Valencia-Hipolito, Alberto
    LONG NON-CODING RNAS IN HUMAN DISEASE, 2016, 394 : 203 - 236
  • [29] Immune response to cold exposure: Role of γδ T cells and TLR2-mediated inflammation
    Vasek, Daniel
    Holicek, Peter
    Galatik, Frantisek
    Kratochvilova, Anna
    Porubska, Bianka
    Somova, Veronika
    Fikarova, Natalie
    Hajkova, Michaela
    Prevorovsky, Martin
    Zurmanova, Jitka M.
    Krulova, Magdalena
    EUROPEAN JOURNAL OF IMMUNOLOGY, 2024, 54 (10)
  • [30] COMPARISON OF THE EFFECTS OF PROLONGED EXPOSURE TO COLD IN THE RAT AND GUINEA-PIG
    FREMINET, A
    FORICHON, J
    DALLEVET, G
    JOMAIN, MJ
    MINAIRE, Y
    JOURNAL DE PHYSIOLOGIE, 1981, 77 (09): : A35 - A35