Biochemical and cellular insights into the Baz2B protein, a non-catalytic subunit of the chromatin remodeling complex

被引：2

作者：

Breindl, Matthias ^{[1
]}

Spitzer, Dominika ^{[1
]}

Gerasimaite, Ruta ^{[2
]}

Kairys, Visvaldas ^{[3
]}

Schubert, Thomas ^{[4
]}

Henfling, Ramona ^{[1
]}

Schwartz, Uwe ^{[5
]}

Lukinavicius, Grazvydas ^{[2
]}

Manelyte, Laura ^{[1
]}

机构：

[1] Univ Regensburg, Biochem 3, DE-93053 Regensburg, Germany

[2] Max Planck Inst Multidisciplinary Sci, Dept NanoBiophoton, Chromatin Labeling & Imaging Grp, Fassberg 11, DE-37077 Gottingen, Germany

[3] Vilnius Univ, Inst Biotechnol, Life Sci Ctr, LT-10257 Vilnius, Lithuania

[4] 2bind GmbH, DE-93053 Regensburg, Germany

[5] Univ Regensburg, NGS Anal Ctr, DE-93053 Regensburg, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2024年 / 52卷 / 01期

关键词：

DNA-BINDING DOMAIN; AT-HOOK MOTIFS; HETEROCHROMATIN FORMATION; MAMMALIAN ISWI; BROMODOMAIN; NORC; RNA; FAMILY; RECOGNITION; STABILITY;

D O I：

10.1093/nar/gkad1096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Baz2B is a regulatory subunit of the ATP-dependent chromatin remodeling complexes BRF1 and BRF5, which control access to DNA during DNA-templated processes. Baz2B has been implicated in several diseases and also in unhealthy ageing, however limited information is available on the domains and cellular roles of Baz2B. To gain more insight into the Baz2B function, we biochemically characterized the TAM (Tip5/ARBP/MBD) domain with the auxiliary AT-hook motifs and the bromodomain (BRD). We observed alterations in histone code recognition in bromodomains carrying cancer-associated point mutations, suggesting their potential involvement in disease. Furthermore, the depletion of Baz2B in the Hap1 cell line resulted in altered cell morphology, reduced colony formation and perturbed transcriptional profiles. Despite that, super-resolution microscopy images revealed no changes in the overall chromatin structure in the absence of Baz2B. These findings provide insights into the biological function of Baz2B. Graphical Abstract

引用

页码：337 / 354

页数：18

共 15 条

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