Imaging of mitochondria/lysosomes in live cells and C. elegans

被引：0
|
作者：
Singh, Deepmala [1 ]
Regar, Ramprasad [1 ]
Soppina, Pushpanjali [2 ,3 ]
Soppina, Virupakshi [2 ]
Kanvah, Sriram [1 ]
机构：
[1] Indian Inst Technol Gandhinagar, Dept Chem, Gandhinagar 382055, Gujarat, India
[2] Indian Inst Technol Gandhinagar, Dept Biol Engn, Gandhinagar 382055, Gujarat, India
[3] Sambalpur Univ, Dept Biotechnol & Bioinformat, Sambalpur 768019, Orissa, India
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2023年 / 21卷 / 10期
关键词：
PHOTODYNAMIC THERAPY; METHYLENE-BLUE; RHODAMINE-B; PHOTOSENSITIZERS; CHALLENGES; DESIGN; PROBES;
D O I：
10.1039/d3ob00086a
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Two rhodamine-phenothiazine conjugates, RP1 and RP2, were synthesized, and their photophysical properties, subcellular localization, and photocytotoxicity were investigated. We observed robust localization of RP1 in mitochondria and dual localization in mitochondria and lysosomes with RP2 in live cells. Live cell imaging with these probes allowed us to track the dynamics of mitochondria and lysosomes during ROS-induced mitochondrial damage and the subsequent lysosomal digestion of the damaged mitochondria. The fluorophores also demonstrated preferential accumulation in cancer cells compared to normal cells and had strong photo-cytotoxicity. However, no cytotoxicity was observed in the dark. The mitochondrial staining and light-induced ROS production were not limited to mammalian cell lines, but were also observed in the animal model C. elegans. The study demonstrated the potential applications of these probes in visualizing the mitochondria-lysosome cross-talk after ROS production and for photodynamic therapy.
引用
收藏
页码：2220 / 2231
页数：12
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