In Situ Stability Test for mRNA Vaccines Based on Deep-UV Resonance Raman Spectroscopy

被引：1
|
作者：
Almehmadi, Lamyaa M. [1 ,2 ]
Reverdatto, Sergei V. [1 ,2 ]
Ermolenkov, Vladimir V. [2 ]
Shekhtman, Alexander [1 ,2 ]
Lednev, Igor K. [1 ,2 ]
机构：
[1] SUNY Albany, Coll Arts & Sci, Dept Chem, Albany, NY 12222 USA
[2] SUNY Albany, RNA Inst, Coll Arts & Sci, Albany, NY 12222 USA
来源：
ANALYTICAL CHEMISTRY | 2023年 / 96卷 / 03期
关键词：
PSEUDOURIDINE;
D O I：
10.1021/acs.analchem.3c01761
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Deep-UV resonance Raman spectroscopy has been shown to offer great potential for probing the in situ stability of mRNA vaccines. In this study, a vaccine model was subjected to controlled degradation using RNase A or through aging at room temperature. The degradation of mRNA was confirmed by using a cell transfection test and by gel electrophoresis. Under both settings, DUVRR spectroscopy successfully revealed the mRNA degradation signs of the vaccine model.
引用
收藏
页码：1003 / 1008
页数：6
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