The use of medicinal plants among local experts and the general population of San Sebastian Coatlan, Oaxaca, Mexico was documented. A total of 117 medicinal species were recorded, 61% of which are native to Mexico, being used to treat 16 types of illnesses. In total, 44.4% of medicinal species can be obtained from home gardens. Local experts and general population contribute similarly to the total knowledge of medicinal plants. In total, 45% of the medicinal plants are herbaceous, and 65% are cultivated. Community traditional knowledge about medicinal plants is distributed and conserved among all members of the locality.