Insights into the Biological Activity and Cytotoxic Mechanism of Epimedium pubigerum

被引：1
|
作者：
Seyhan, Gokce [1 ]
Akkaya, Didem [1 ]
Kolci, Kubra [2 ,3 ]
Reis, Rengin [2 ]
Yazici, Nurdan [4 ]
Barut, Elif Nur [5 ]
Barut, Burak [1 ]
机构：
[1] Karadeniz Tech Univ, Fac Pharm, Biochem Dept, TR-61080 Trabzon, Turkiye
[2] Acibadem Univ, Fac Pharm, Pharmaceut Toxicol Dept, TR-34752 Istanbul, Turkiye
[3] Yeditepe Univ, Fac Pharm, Pharmaceut Toxicol Dept, TR-34755 Istanbul, Turkiye
[4] Karadeniz Tech Univ, Fac Pharm, Pharmacognosy Dept, TR-61080 Trabzon, Turkiye
[5] Karadeniz Tech Univ, Fac Pharm, Pharmacol Dept, TR-61080 Trabzon, Turkiye
来源：
CHEMISTRY & BIODIVERSITY | 2023年 / 20卷 / 11期
关键词：
apoptosis; Epimedium pubigerum; lung cancer; LC-HRMS; p53; PHOTODYNAMIC THERAPY; LUNG-CANCER; APOPTOSIS; INDUCTION; EXTRACTS; GROWTH;
D O I：
10.1002/cbdv.202301003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work, the phytochemical characterization, biological activity, and cytotoxic mechanism of aerial and rhizome methanol extracts (SME and RME) of Epimedium pubigerum were investigated to demonstrate its potential usage in the treatment of lung cancer. LC-HRMS analysis, total phenolic/flavonoid content assay, DPPH radical scavenging assay, DNA interaction, cytotoxicity, and western blotting were investigated using different methods. Fumaric acid was found to be the most abundant compound in both extracts. SME and RME were cytotoxic on A549 cells concentration-dependently. Also, in vitro scratch assay showed that SME and RME led to a significant anti-migratory effect at 1 mg/mL. Cytochrome c, p53, and caspase 3 expression significantly increased in the presence of RME compared to the control. All of these results claimed that RME might be suggested as a theoretically more effective phytotherapeutic agent for lung cancer compared to the effect seen with the SME.
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页数：11
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