Water and Molecular Exchange in Biological Cells Studied Using 1H Pulsed Field Gradient NMR

被引:1
|
作者
Avilova, Irina A. A. [1 ]
Volkov, Vitaly I. I. [1 ,2 ]
机构
[1] Fed Res Ctr Problems Chem Phys & Med Chem RAS, Chernogolovka 142432, Russia
[2] Inst Solid State Phys RAS, Sci Ctr Chernogolovka, Chernogolovka 142432, Russia
关键词
biological cell; biological membrane; pulsed field gradient NMR; diffusion; permeability; lipid bilayers; lateral diffusion; SELF-DIFFUSION COEFFICIENT; LIPID LATERAL DIFFUSION; MEMBRANE-PERMEABILITY; HUMAN ERYTHROCYTES; INTRACELLULAR WATER; MAGNETIC-FIELD; RED-CELLS; PGSE NMR; CHOLESTEROL; SUSPENSIONS;
D O I
10.3390/membranes13060567
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: the extraction of self-diffusion coefficients, calculation of cell sizes, and permeability of cell membranes. Attention is paid to the results of assessing the permeability of biological membranes for water molecules and biologically active compounds. The results for other systems are also presented: yeast, chlorella, and plant cells. The results of studies of the lateral diffusion of lipid and cholesterol molecules in model bilayers are also presented.
引用
收藏
页数:19
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