This study aimed to evaluate the effect of mastic specifications on the intermediate and low temperatures performance of hot mix asphalt. In this research study, four different asphalt mixtures were fabricated with two types of aggregate and bitumen at three different mastic densities (defined as F/B ratios). At intermediate temperature, Four-Point Bending (4PB) beam fatigue tests were carried out at 8 Hz frequency and the strain levels of 460, 750, and 1100 microstrain. Also, the low-temperature performance of mixtures was evaluated by using Semi-Circular Bending (SCB) tests at -6 degrees C, -18 degrees C and -12 degrees C, -24 degrees C for PG 64-16 and PG 58-22 fabricated mixtures, respectively. Fatigue test results showed that the fatigue life of asphalt mixtures was more influenced by the mastic density at the lower strain level. Moreover, a minimum mastic density was necessary for improving fatigue performance. The SCB test results revealed that increasing the mastic density makes higher values in the mixture's fracture energy, fracture toughness, cracking resistance index (CRI), and tensile strength. Also, the low-temperature performance of asphalt mixtures containing aggregate with a high abrasion value (AAV) was more dependent on the mastic density.