Baculovirus Actin Rearrangement-Inducing Factor 1 Can Remodel the Mammalian Actin Cytoskeleton

被引:2
|
作者
Steffen, Anika [1 ]
Reusch, Bjoern [2 ,5 ]
Gruteser, Nadine [3 ]
Mainz, Daniela [2 ]
Roncarati, Renza [2 ,6 ]
Baumann, Arnd [3 ]
Stradal, Theresia E. B. [1 ]
Knebel-Moersdorf, Dagmar [2 ,4 ]
机构
[1] Helmholtz Ctr Infect Res, Dept Cell Biol, Braunschweig, Germany
[2] Univ Cologne, Univ Hosp Cologne, Ctr Biochem, Cologne, Germany
[3] Res Ctr Juelich, Inst Biol Informat Proc Mol & Cellular Physiol, Julich, Germany
[4] Univ Cologne, Univ Hosp Cologne, Dept Pediat, Cologne, Germany
[5] Univ Cologne, Univ Hosp Cologne, Inst Human Genet, Cologne, Germany
[6] Informat Technol Translat Med, Esch Sur Alzette, Luxembourg
关键词
Arif-1; AcMNPV; F-actin remodeling; actin dynamics; B16-F1; cells; TN368; NUCLEAR POLYHEDROSIS-VIRUS; F-ACTIN; INFECTION; CELLS; IDENTIFICATION; LOCALIZATION; COLOCALIZES; PE38; IE2;
D O I
10.1128/spectrum.05189-22
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Virus-induced changes of the host cell cytoskeleton play a pivotal role in the pathogenesis of viral infections. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is known for intervening with the regulation of the host actin cytoskeleton in a wide manner throughout the infection cycle. The actin rearrangement-inducing factor 1 (Arif-1) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an early viral protein that manipulates the actin cytoskeleton of host insect cells. Arif-1 is conserved among alphabaculoviruses and is responsible for the accumulation of F-actin at the plasma membrane during the early phase of infection. However, the molecular mechanism underlying Arif-1-induced cortical actin accumulation is still open. Recent studies have demonstrated the formation of invadosome-like structures induced by Arif-1, suggesting a function in systemic virus spread. Here, we addressed whether Arif-1 is able to manipulate the actin cytoskeleton of mammalian cells comparably to insect cells. Strikingly, transient overexpression of Arif-1 in B16-F1 mouse melanoma cells revealed pronounced F-actin remodeling. Actin assembly was increased, and intense membrane ruffling occurred at the expense of substrate-associated lamellipodia. Deletion mutagenesis studies of Arif-1 confirmed that the C-terminal cytoplasmic region was not sufficient to induce F-actin remodeling, supporting that the transmembrane region for Arif-1 function is also required in mammalian cells. The similarities between Arif-1-induced actin remodeling in insect and mammalian cells indicate that Arif-1 function relies on conserved cellular interaction partners and signal transduction pathways, thus providing an experimental tool to elucidate the underlying mechanism. IMPORTANCE Virus-induced changes of the host cell cytoskeleton play a pivotal role in the pathogenesis of viral infections. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is known for intervening with the regulation of the host actin cytoskeleton in a wide manner throughout the infection cycle. The actin rearrangement-inducing factor 1 (Arif-1) is a viral protein that causes actin rearrangement during the early phase of AcMNPV infection. Here, we performed overexpression studies of Arif-1 in mammalian cells to establish an experimental tool that allows elucidation of the mechanism underlying the Arif-1-induced remodeling of actin dynamics in a well-characterized and genetically accessible system.
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页数:11
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