The spatiotemporal changes of spatial conflict not only reflect changes in the structure of regional ecosystems, but also have an impact on regional ecological security. Taking the Yancheng Coastal Wetland as a case and using remote sensing images in 1997, 2007, and 2017 as data sources, a spatial conflict model was selected from three dimensions of external pressure, landscape vulnerability and landscape stability. The spatiotemporal changes of spatial conflict were analyzed based on landscape patterns. From 1997 to 2017, influenced by human activities, the landscape structure and pattern underwent significant changes in the Yancheng coastal wetlands, with a significant reduction in natural wetlands. The external pressure showed a characteristic of first decreasing and then increasing, with a low level of spatial differentiation. The landscape vulnerability first decreased and then increased, with significant spatial differentiation in the sea-land direction. The landscape stability also first decreased and then increased, with a low level of spatial differentiation. The spatial conflict first increased and then decreased, the mean values were 0.356, 0.406, 0.380 in 1997, 2007 and 2017 respectively, at the levels of stable and controllable and basically controllable level, with significant spatial differentiation in the sealand direction. There were significant differences in the level of spatial conflict among different landscape types, with natural wetlands facing the largest spatial conflict and human production and living land having smaller spatial conflicts. This research can provide theoretical reference for creating a model of harmonious development between humans and nature in Yancheng.