Study on Separation Mechanism of Ephedrine and Pseudoephedrine in Borax Buffer by Capillary Electrophoresis

被引：1

作者：

Xu, Jinqiu ^{[1
]}

Gong, Yinhua ^{[2
]}

Huang, Rongrong ^{[1
]}

Cai, Liangliang ^{[1
]}

Ma, Xiaofei ^{[1
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Dept Pharm, Nantong 226001, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Pharm, Suzhou 215006, Peoples R China

来源：

CURRENT ANALYTICAL CHEMISTRY | 2023年 / 19卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Borax; ephedrine; pseudoephedrine; capillary electrophoresis; complexation; separation mechanism; CHIRAL SELECTOR; BASIC DRUGS; COMPLEXATION; ENANTIOSEPARATION; SILICA;

D O I：

10.2174/0115734110270003231101115858

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Background: In borax buffer, ephedrine (EPH) and pseudoephedrine (PSE) can be well separated (Rs>10) in capillary electrophoresis (CE) without adding any other compounds, but the specific mechanism remains unclear.Methods: Titration analysis, nuclear magnetic resonance analysis, mass spectrum analysis and molecular modeling were used to investigate the separation mechanism.Results; Tetrahydroxy borate (a hydrolyzate of borax or boric acid) could react with ephedrine and pseudoephedrine to form self-assembled complexes, but the two complexes had different stabilities and electrophoretic mobilities resulting from the different steric configurations of analytes.Conclusion: The complexation based on borax was responsible for the separation.

引用

页码：685 / 692

页数：8

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