Study on Separation Mechanism of Ephedrine and Pseudoephedrine in Borax Buffer by Capillary Electrophoresis

被引：1
|
作者：
Xu, Jinqiu [1 ]
Gong, Yinhua [2 ]
Huang, Rongrong [1 ]
Cai, Liangliang [1 ]
Ma, Xiaofei [1 ]
机构：
[1] Nantong Univ, Affiliated Hosp, Dept Pharm, Nantong 226001, Peoples R China
[2] Soochow Univ, Affiliated Hosp 1, Dept Pharm, Suzhou 215006, Peoples R China
来源：
CURRENT ANALYTICAL CHEMISTRY | 2023年 / 19卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Borax; ephedrine; pseudoephedrine; capillary electrophoresis; complexation; separation mechanism; CHIRAL SELECTOR; BASIC DRUGS; COMPLEXATION; ENANTIOSEPARATION; SILICA;
D O I：
10.2174/0115734110270003231101115858
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Background: In borax buffer, ephedrine (EPH) and pseudoephedrine (PSE) can be well separated (Rs>10) in capillary electrophoresis (CE) without adding any other compounds, but the specific mechanism remains unclear.Methods: Titration analysis, nuclear magnetic resonance analysis, mass spectrum analysis and molecular modeling were used to investigate the separation mechanism.Results; Tetrahydroxy borate (a hydrolyzate of borax or boric acid) could react with ephedrine and pseudoephedrine to form self-assembled complexes, but the two complexes had different stabilities and electrophoretic mobilities resulting from the different steric configurations of analytes.Conclusion: The complexation based on borax was responsible for the separation.
引用
收藏
页码：685 / 692
页数：8
相关论文
共 50 条
  • [1] The ephedrine and pseudoephedrine separation with the capillary electrophoresis and determination by conductivity detector
    Zheng, YN
    Xie, TY
    Mo, JY
    Wei, SL
    [J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2002, 23 (02): : 199 - 202
  • [2] The Ephedrine and Pseudoephedrine Separation with the Capillary Electrophoresis and Determination by Conductivity Detector
    Zheng, Yi-Ning
    Xie, Tian-Yao
    Mo, Jin-Yuan
    Wei, Shou-Lian
    [J]. Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities, 2002, 23 (02):
  • [3] Separation and determination of ephedrine and pseudoephedrine by combination of flow injection with capillary electrophoresis
    Chen, HL
    Chen, XG
    Pu, QS
    Hu, ZD
    Zhao, ZF
    Hooper, M
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2003, 41 (01) : 1 - 5
  • [4] Separation of enantiomeric ephedrine and pseudoephedrine - High pressure liquid chromatography and capillary electrophoresis
    Iwanicki, RM
    Maier, K
    Zlotnick, JA
    Liu, RH
    Kuo, TS
    Tagliaro, F
    [J]. JOURNAL OF FORENSIC SCIENCES, 1999, 44 (03) : 470 - 474
  • [5] Application of Capillary Electrophoresis for Ephedrine and Pseudoephedrine Detection: a Review
    Sun, Yu
    Zhang, Shuai
    [J]. INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2020, 15 (03): : 2614 - 2623
  • [6] DETERMINATION OF EPHEDRINE AND PSEUDOEPHEDRINE IN CHINESE HERBAL PREPARATIONS BY CAPILLARY ELECTROPHORESIS
    LIU, YM
    SHEU, SJ
    [J]. JOURNAL OF CHROMATOGRAPHY, 1993, 637 (02): : 219 - 223
  • [7] The use of capillary zone electrophoresis for analysis of ephedrine and pseudoephedrine in food supplements
    Jelínek, I
    [J]. CHEMICKE LISTY, 1999, 93 (12): : 800 - 802
  • [8] Chiral separation of ephedrine isomers by capillary electrophoresis using bovine serum albumin as a buffer additive
    Ye, Nengsheng
    Gu, Xuexin
    Luo, Guoan
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2007, 45 (05) : 246 - 250
  • [9] Determination of Ephedrine and Pseudoephedrine by Field-Amplified Sample Injection Capillary Electrophoresis
    Deng, Dongli
    Deng, Hao
    Zhang, Lichun
    Su, Yingying
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2014, 52 (04) : 357 - 362
  • [10] Sensitive determination of ephedrine and pseudoephedrine by capillary electrophoresis with laser-induced fluorescence detection
    Zhang, JY
    Xie, JP
    Chen, XG
    Hu, ZD
    [J]. ANALYST, 2003, 128 (04) : 369 - 372
12345