Decoding the microbial universe with metagenomics: a brief insight

被引:3
|
作者
Chaudhari, Hiral G. [1 ]
Prajapati, Shobha [2 ]
Wardah, Zuhour Hussein [1 ]
Raol, Gopal [3 ]
Prajapati, Vimalkumar [4 ]
Patel, Rajesh [2 ]
Shati, Ali A. [5 ]
Alfaifi, Mohammad Y. [5 ]
Elbehairi, Serag Eldin I. [5 ]
Sayyed, R. Z. [6 ]
机构
[1] Sardar Patel Univ, Shri Alpesh N Patel PG Inst Sci & Res, Anand, Gujarat, India
[2] Veer Narmad South Gujarat Univ, Dept Biosci, Surat, Gujarat, India
[3] Shri RP Arts Shri KB Commerce & Smt BCJ Sci Coll, Khambhat, Gujarat, India
[4] Navsari Agr Univ, Aspee Shakilam Biotechnol Inst, Div Microbial & Environm Biotechnol, Surat, Gujarat, India
[5] King Khalid Univ, Fac Sci, Biol Dept, Abha, Saudi Arabia
[6] PSGVP Mandals SI Patil Arts GB Patel Sci & STKV Sa, Dept Microbiol, Shahada, India
关键词
NGS; next-generation sequencing; metagenomics; microbial diversity; microbiome; shotgun sequencing; amplicon sequencing; SINGLE-MOLECULE; GUT MICROBIOTA; DNA; DIVERSITY; COMMUNITIES; SEQUENCE; GENOME; IDENTIFICATION; CULTIVATION; MODULATION;
D O I
10.3389/fgene.2023.1119740
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A major part of any biological system on earth involves microorganisms, of which the majority are yet to be cultured. The conventional methods of culturing microbes have given fruitful outcomes yet have limitations. The curiosity for better understanding has led to the development of culture-independent molecular methods that help push aside the roadblocks of earlier methods. Metagenomics unifies the scientific community in search of a better understanding of the functioning of the ecosystem and its component organisms. This approach has opened a new paradigm in advanced research. It has brought to light the vast diversity and novelty among microbial communities and their genomes. This review focuses on the development of this field over time, the techniques and analysis of data generated through sequencing platforms, and its prominent interpretation and representation.
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页数:15
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