Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells

被引：13
|
作者：
Chu, Pei-Yu [1 ]
Hsieh, Han-Yu [1 ,3 ]
Chung, Pei-Shan [2 ]
Wang, Pai-We [3 ]
Wu, Ming-Chung [4 ]
Chen, Yin-Quan [5 ]
Kuo, Jean-Chen [4 ,5 ]
Fan, Yu-Jui [1 ,6 ]
机构：
[1] Taipei Med Univ, Coll Biomed Engn, 250 Wuxing St, Taipei 11031, Taiwan
[2] Univ Calif Los Angeles, Dept Bioengn, 420 Westwood Plaza, Los Angeles, CA 90095 USA
[3] Natl Taiwan Univ, Inst Appl Mech, 1,Sec 4,Roosevelt Rd, Taipei 10617, Taiwan
[4] Natl Yang Ming Chiao Tung Univ, Inst Biochem & Mol Biol, 155,Sec 2,Linong St, Taipei 11221, Taiwan
[5] Natl Yang Ming Chiao Tung Univ, Canc Progress Res Ctr, 155,Sec 2,Linong St, Taipei 11221, Taiwan
[6] Taipei Med Univ, Int PhD Program Biomed Engn, 250 Wuxing St, Taipei 11031, Taiwan
来源：
ISCIENCE | 2023年 / 26卷 / 06期
关键词：
FLUID SHEAR-STRESS; CYCLIC STRETCH; MODULATION; ACTIVATION; INTEGRINS; STRAIN; PDMS; FLOW;
D O I：
10.1016/j.isci.2023.106927
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.
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页数：15
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