Development of Electroanalytical Methods Using Different Electrodes for the Determination of Progesterone (P4) in Pharmaceutical Formulations and Human, Cow and Goat Milk

For the determination of progesterone (P4) electroanalytical studies were performed using mercury electrode (HMDE) and screen-printed boron-doped diamond electrode (BDD-SPE). The effects of pH, supporting electrolyte, accumulation potential and time (E-acc, t(acc)) were studied in both systems. The optimum conditions were: i) HMDE: pH 7,0; -0,1 V and t(acc): 40 s, ii) BDD-SPE: 0,5 mol L-1 H2S ; E-acc: 0,0 V and t(acc): 120 s. Under these conditions, the detection limit was 3.1 mu g L-1 for HMDE and 45.6 mu g L-1 for BDD-SPE. Both methods were validated and applied in the P4 determination on pharmaceutical formulations and milk samples.