Effects of citrate-stabilized gold and silver nanoparticles on some safety parameters of Porphyridium cruentum biomass

被引:4
|
作者
Rudi, Ludmila [1 ]
Cepoi, Liliana [1 ]
Chiriac, Tatiana [1 ]
Miscu, Vera [1 ]
Valuta, Ana [1 ]
Djur, Svetlana [1 ]
机构
[1] Tech Univ Moldova, Inst Microbiol & Biotechnol, Phycobiotechnol Lab, Kishinev, Moldova
关键词
gold; silver; nanoparticles; Porphyridium cruentum; biomass; lipids; malondialdehyde; antioxidant activity; OXIDATIVE STRESS; CHLORELLA; ECOTOXICITY; MICROALGAE; TOXICITY;
D O I
10.3389/fbioe.2023.1224945
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Introduction: Our research raises the question of how realistic and safe it is to use gold and silver nanoparticles in biotechnologies to grow microalgae, which will later be used to obtain valuable products. To this purpose, it was necessary to assess the influence of 10 and 20 nm Au and Ag nanoparticles stabilized in citrate on the growth of microalga Porphyridium cruentum in a closed cultivation system, as well as some safety parameters of biomass quality obtained under experimental conditions.Methods: Two types of experiments were conducted with the addition of nanoparticles on the first day and the fifth day of the cultivation cycle. Changes in productivity, lipid content, malondialdehyde (MDA), as well as antioxidant activity of microalgae biomass have been monitored in dynamics during the life cycle in a closed culture system.Results: The impact of nanoparticles on the growth curve of microalgae culture was marked by delaying the onset of the exponential growth phase. A significant increase in the content of lipids and MDA in biomass was noted. Excessive accumulation of lipid oxidation products within the first 24 h of cultivation resulted in altered antioxidant activity of red algae extracts.Discussion: Citrate-stabilized gold and silver nanoparticles proved to be a stress factor for red microalga Porphyridium cruentum, causing significant changes in both biotechnological and biomass safety parameters. Addition of Au and Ag nanoparticles during the exponential growth phase of porphyridium culture led to an enhanced lipid accumulation and reduced MDA values in biomass.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Signatures of specificity of interactions of binary protein mixtures with citrate-stabilized gold nanoparticles
    Khandelia, Rumi
    Deka, Jashmini
    Paul, Anumita
    Chattopadhyay, Arun
    RSC ADVANCES, 2012, 2 (13): : 5617 - 5628
  • [22] The effect of hardness on the stability of citrate-stabilized gold nanoparticles and their uptake by Daphnia magna
    Lee, Byung-Tae
    Ranville, James F.
    JOURNAL OF HAZARDOUS MATERIALS, 2012, 213 : 434 - 439
  • [23] The Effect of Aniline Concentration in the Ligand Exchange Reaction with Citrate-Stabilized Gold Nanoparticles
    Newman, Janelle D. S.
    MacCrehan, William A.
    LANGMUIR, 2009, 25 (16) : 8993 - 8998
  • [24] Synthesis of Citrate-Stabilized Silver Nanoparticles Modified by Thermal and pH Preconditioned Tannic Acid
    La Spina, Rita
    Mehn, Dora
    Fumagalli, Francesco
    Holland, Margaret
    Reniero, Fabiano
    Rossi, Francois
    Gilliland, Douglas
    NANOMATERIALS, 2020, 10 (10) : 1 - 16
  • [25] Visual colorimetric detection of cyromazine in river water using citrate-stabilized gold nanoparticles
    Bai, Wenhui
    Zhu, Chao
    Zhang, Guilan
    Huang, Yafei
    Yan, Jiao
    Yan, Mengmeng
    Chen, Ailiang
    ANALYTICAL METHODS, 2016, 8 (29) : 5869 - 5873
  • [26] Decreased resonance light scattering of citrate-stabilized gold nanoparticles by chemisorption of mercaptoacetic acid
    Weng, Guojun
    Li, Jianjun
    Zhu, Jian
    Zhao, Junwu
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2010, 369 (1-3) : 253 - 259
  • [27] Citrate-stabilized gold nanoparticles hinder fibrillogenesis of a pathological variant of β2-microglobulin
    Cantarutti, Cristina
    Raimondi, Sara
    Brancolini, Giorgia
    Corazza, Alessandra
    Giorgetti, Sofia
    Ballico, Maurizio
    Zanini, Stefano
    Palmisano, Giovanni
    Bertoncin, Paolo
    Marchese, Loredana
    Mangione, P. Patrizia
    Bellotti, Vittorio
    Corni, Stefano
    Fogolari, Federico
    Esposito, Gennaro
    NANOSCALE, 2017, 9 (11) : 3941 - 3951
  • [28] Colorimetric detection of quaternary ammonium surfactants using citrate-stabilized gold nanoparticles (Au NPs)
    Zheng, Li-Qing
    Yu, Xiao-Dong
    Xu, Jing-Juan
    Chen, Hong-Yuan
    ANALYTICAL METHODS, 2014, 6 (07) : 2031 - 2033
  • [29] Iron-Assisted Synthesis of Highly Monodispersed and Magnetic Citrate-Stabilized Small Silver Nanoparticles
    Blanco-Formoso, Maria
    Turino, Mariacristina
    Rivas-Murias, Beatriz
    Guerrini, Luca
    Shave, Alexey
    de la Rica, Roberto
    Correa-Duarte, Miguel
    Salgueirino, Veronica
    Pazos-Perez, Nicolas
    Alvarez-Puebla, Ramon A.
    JOURNAL OF PHYSICAL CHEMISTRY C, 2020, 124 (05): : 3270 - 3276
  • [30] Kinetic analysis of the hydrolysis of methyl parathion using citrate-stabilized 10 nm gold nanoparticles
    Nita, Rafaela
    Trammell, Scott A.
    Ellis, Gregory A.
    Moore, Martin H.
    Soto, Carissa M.
    Leary, Dagmar H.
    Fontana, Jake
    Talebzadeh, Somayeh F.
    Knight, D. Andrew
    CHEMOSPHERE, 2016, 144 : 1916 - 1919