A DIGITAL PCR CHIP WITH 3D STRUCTURE AND COMPOSITE MATERIALS

被引：0
|
作者：
Gao, Shiyuan [1 ,2 ,3 ]
Xu, Tiegang [1 ,2 ]
Wu, Lei [1 ,2 ]
Zhu, Xiaoyue [4 ]
Ma, Zhan [5 ]
Li, Xinxin [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China
[2] Univ Chinese Acad Sci, Coll Mat Sci & Opto Elect Technol, Beijing 100049, Peoples R China
[3] ShanghaiTech Univ, Sch Informat Sci & Technol, Shanghai 201210, Peoples R China
[4] Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Sch Future Technol, Metabol Ctr, Fuzhou 350002, Fujian, Peoples R China
[5] Shanghai Jiao Tong Univ, Shanghai Childrens Hosp, Fac Med Lab Sci, Sch Med, Shanghai 200062, Peoples R China
来源：
2024 IEEE 37TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2024年
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
Digital PCR; silicon; three-dimensional; high-density; fluorescent light reflection;
D O I：
10.1109/MEMS58180.2024.10439396
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We designed and fabricated a three-dimensional (3D) digital PCR (dPCR) chip for better sample compartmentalization and stronger fluorescent signals. The chip was made of glass, silicon and poly dimethylsiloxane (PDMS) layers, respectively. All the microstructures were fabricated on the silicon substrate, with high-density hexagonal-shape reaction units regularly arranged in the reaction zone. A common fed channel was located underneath the reaction units to maximize the use of the chip area. The reaction units and the flow channel were connected by cylindrical micro-holes, which made the oil-water two-phase system more stable and reduced the interference of fluorescence signals due to residual sample in the flow channel. In addition, fluorescent light reflection on the silicon surface enhanced the resolution of dPCR image.
引用
收藏
页码：665 / 668
页数：4
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