DUSP4 maintains the survival and LSD1 protein stability in esophageal squamous cell carcinoma cells by inhibiting JNK signaling-dependent autophagy

被引:0
|
作者
Liu, Xinxin [1 ,2 ]
Ye, Zhou [3 ]
Rao, Dingyu [4 ]
Chen, Qianshun [5 ]
Zhang, Zuxiong [4 ,5 ]
机构
[1] Gannan Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Ganzhou 341000, Jiangxi, Peoples R China
[2] Gannan Med Univ, Garman Branch, Ctr Natl Geriatr Dis Clin Med Res Ctr, Ganzhou 341000, Jiangxi, Peoples R China
[3] 900th Hosp Joint Logist Support Force PLA, Dept Cardiol, Fuzhou, Fujian, Peoples R China
[4] Gannan Med Univ, Dept Gynecol & Obstet, Affiliated Hosp 1, 23 Qingnian Rd, Ganzhou 341000, Jiangxi, Peoples R China
[5] Fujian Med Univ, Shengli Clin Med Coll, Fuzhou 350001, Fujian, Peoples R China
关键词
MAP-KINASE PHOSPHATASE; CANCER; ACTIVATION; PHOSPHORYLATION; OVEREXPRESSION; BCL-2; EXPRESSION; APOPTOSIS; PATHWAYS; ARREST;
D O I
10.1007/s11626-023-00845-4
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
DUSP4 is a biomarker of esophageal squamous cell carcinoma (ESCC), which is responsible for the prognosis in ESCC. However, the underlying mechanism of DUSP4-regulated ESCC carcinogenesis is unknown. As a negative regulator of JNK, DUSP4 can inhibit autophagy, which contributes to tumorigenesis. This study aimed to explore the role of autophagy in DUSP4-regulated ESCC carcinogenesis. Our results showed that DUSP4 overexpression inhibited autophagy and promoted LSD1 protein expression in ESCC cells, while DUSP4 silencing showed the opposite effects. However, DUSP4 overexpression and silencing did not affect LSD1 mRNA expression. But the regulatory ability of DUSP4 overexpression on autophagy, death level, and LSD1 protein was reversed by rapamycin. In addition, DUSP4 overexpression inhibited JNK and Bcl2 phosphorylation and the dissociation of Bcl2-Beclin1 complex, while DUSP4 silencing promoted JNK and Bcl2 phosphorylation. Moreover, the regulatory ability of DUSP4 overexpression on autophagy, death, and LSD1 protein was reversed by JNK activator anisomycin. The xenograft assays also showed that DUSP4 overexpression-promoted ESCC tumor growth in vivo and LC3II and LSD1 protein expression in tumor tissues were reversed by rapamycin or anisomycin. Overall, DUSP4 inhibits Bcl2-Beclin1-autophagy signal transduction through the negative regulation of JNK, thus suppressing autophagic death and the autophagic degradation of LSD1 in ESCC, by which DUSP4 promotes ESCC carcinogenesis.
引用
收藏
页码:115 / 122
页数:8
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