Decreased expression of TFF2 and decreased αGlcNAc glycosylation are malignant biomarkers of pyloric gland adenoma of the duodenum

被引：1

作者：

Yamanoi, Kazuhiro ^{[1
,2
]}

Fujii, Chifumi ^{[2
,3
,4
]}

Nakayama, Atsushi ^{[5
]}

Matsuura, Noriko ^{[5
]}

Takatori, Yusaku ^{[5
]}

Kato, Motohiko ^{[5
]}

Yahagi, Naohisa ^{[5
]}

Nakayama, Jun ^{[2
]}

机构：

[1] Keio Univ, Sch Med, Dept Pathol, 35 Shinanomachi,Shinjuku, Tokyo 1608582, Japan

[2] Shinshu Univ, Dept Mol Pathol, Sch Med, Matsumoto, Japan

[3] Shinshu Univ, Inst Biomed Sci, Dept Biotechnol, Interdisciplinary Cluster Cutting Edge Res, Matsumoto, Japan

[4] Shinshu Univ, Ctr Med Educ & Clin Training, Sch Med, Matsumoto, Japan

[5] Keio Univ, Sch Med, Canc Ctr, Div Res & Dev Minimally Invas Treatment, Tokyo, Japan

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

基金：

日本学术振兴会;

关键词：

TREFOIL FACTORS; GASTRIC ADENOCARCINOMA; O-GLYCAN; MUCIN; PROGRESSION; ENTITY; LECTIN; FORMS;

D O I：

10.1038/s41598-023-49040-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pyloric gland adenoma (PGA) is a duodenal neoplasm expressing MUC6 and is often associated with high-grade dysplasia and adenocarcinoma. MUC6 secreted from the pyloric gland cells carries unique O-glycans exhibiting terminal alpha 1,4-linked N-acetylglucosamine residues (alpha GlcNAc). The small peptide trefoil factor 2 (TFF2) is also secreted from pyloric gland cells and binds to alpha GlcNAc. We recently demonstrated that alpha GlcNAc serves as a tumor suppressor for gastric neoplasm including PGA, but the significance of TFF2 expression remains unknown. We examined 20 lesions representing low- and high-grade PGA in 22 cases by immunohistochemistry for alpha GlcNAc, TFF2, MUC6, MUC5AC, MUC2 and p53. alpha GlcNAc, TFF2 and MUC6 were co-expressed on the cell surface and a dot-like pattern in the cytosol in low-grade PGA lesions. High-grade PGA also expressed MUC6, but reduced alpha GlcNAc and TFF2 expression. The ratios of alpha GlcNAc or TFF2 to MUC6 score in high-grade PGA were significantly lower than low-grade PGA (P < 0.001). Co-expression of alpha GlcNAc-glycosylated MUC6 and TFF2 in PGA suggests the existence of alpha GlcNAc/TFF2 form complex in PGA cells, a finding consistent with our observations in non-neoplastic Brunner's gland cells. The decreased alpha GlcNAc and TFF2 expression are associated with high grade atypical cells, indicative of the malignant potential of PGA.

引用

页数：10