Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311

被引:5
|
作者
Xu, Zuopeng [1 ,2 ]
Du, Yuanyue [1 ]
Li, Xixu [1 ]
Wang, Ruixuan [1 ]
Chen, Zhi'ai [1 ]
Zhao, Xiangqiang [3 ]
Liu, Qiaoquan [1 ,2 ]
Tang, Shuzhu [1 ,2 ]
Zhang, Honggen [1 ]
机构
[1] Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Key Lab Plant Funct Genom,Minist Educ, Agr Coll,Jiangsu Key Lab Crop Genom & Mol Breeding, Yangzhou 225009, Jiangsu, Peoples R China
[2] Yangzhou Univ, Jiangsu Co Innovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China
[3] Nantong Univ, Sch Life Sci, Nantong 226019, Jiangsu, Peoples R China
来源
CROP JOURNAL | 2023年 / 11卷 / 03期
基金
中国国家自然科学基金;
关键词
Rice; WA-type CMS; Restorer of fertility ( Rf ) gene; Gene mapping; RESTORATION; MITOCHONDRIAL; PROTEIN; SYSTEM; LOCI; CMS; EXPRESSION; ENCODES; MARKERS;
D O I
10.1016/j.cj.2022.11.009
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The wild abortive (WA)-type cytoplasmic male sterility (CMS) derived from the wild rice species Oryza rufipogon Griff. is used widely in three-line indica hybrids. The identification and mapping of restorer of fertility (Rf) genes aided in the development of WA-type hybrids. Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set, indicating that 9311 carries minor-effect Rfs for WA-type CMS. We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WA-TianfengA genetic background with moderate seed setting rates. Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19 (t). By use of the RICE 40 K gene chip, three introduced segments were identified in the fertile lines, and a candidate region spanning 4.37-8.29 Mb on chromosome 1 was identified for Rf19(t). Finally, Rf19(t) was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183, in which eight ORFs were predicted. Also, using relative expression analyses, comparative sequence analyses and func-tional domain analyses, we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t). Furthermore, Rf19(t) was found to function in fertility restoration, most probably by regulating the degra-dation of mRNA transcribed from the mitochondrial gene WA352. These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WA-type CMS and maintainer lines.& COPY; 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:887 / 894
页数:8
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