Demonstration of seed inoculation of dimer infectious clones of mungbean yellow mosaic India virus-soybean isolate

被引：0
|
作者：
Gupta, Taruna [1 ]
Singhal, Tripti [1 ]
Dhir, Sunny [2 ]
Chandel, Vanita [1 ]
Rishi, Narayan [1 ]
Raj, S. K. [3 ]
Srivastava, Ashish [1 ,4 ]
机构：
[1] Amity Univ Uttar Pradesh, Amity Inst Virol & Immunol, Noida 201313, India
[2] Maharishi Markandeshwar, Dept Biosci & Technol, Cent Res Cell, Ambala 133207, India
[3] B1-66,Sect G, Lucknow 226021, India
[4] Univ Arkansas Syst, Dept Entomol & Plant Pathol, Div Agr, Fayetteville, AR 72701 USA
来源：
3 BIOTECH | 2023年 / 13卷 / 11期
关键词：
Agroinfectious clones; Bipartite begomovirus; Seed inoculation; Detection primers;
D O I：
10.1007/s13205-023-03778-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.
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页数：5
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