"Not-so-popular" orthogonal pairs in genetic code expansion

被引：12
|
作者：
Andrews, Joseph [1 ]
Gan, Qinglei [1 ]
Fan, Chenguang [1 ,2 ,3 ]
机构：
[1] Univ Arkansas, Dept Chem & Biochem, Fayetteville, AR USA
[2] Univ Arkansas, Cell & Mol Biol Program, Fayetteville, AR USA
[3] Dept Chem & Biochem, CHEM 119,345 N Campus Dr, Fayetteville, AR 72701 USA
来源：
PROTEIN SCIENCE | 2023年 / 32卷 / 02期
基金：
美国国家卫生研究院;
关键词：
aminoacyl-tRNA synthetases; genetic code expansion; noncanonical amino acids; orthogonal translation systems; tRNA; TRANSFER-RNA SYNTHETASE; SITE-SPECIFIC INCORPORATION; AMINO-ACID MUTAGENESIS; LEUCYL-TRANSFER-RNA; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; RATIONAL DESIGN; ACCEPTOR STEM; ENZYME; EVOLUTION;
D O I：
10.1002/pro.4559
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
During the past decade, genetic code expansion has been proved to be a powerful tool for protein studies and engineering. As the key part, a series of orthogonal pairs have been developed to site-specifically incorporate hundreds of noncanonical amino acids (ncAAs) into proteins by using bacteria, yeast, mammalian cells, animals, or plants as hosts. Among them, the pair of tyrosyl-tRNA synthetase/tRNA(Tyr) from Methanococcus jannaschii and the pair of pyrrolysyl-tRNA synthetase/tRNA(Pyl) from Methanosarcina species are the most popular ones. Recently, other "not-so-popular" orthogonal pairs have started to attract attentions, because they can provide more choices of ncAA candidates and are necessary for simultaneous incorporation of multiple ncAAs into a single protein. Here, we summarize the development and applications of those "not-so-popular" orthogonal pairs, providing guidance for studying and engineering proteins.
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页数：12
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