Role of heat shock protein 70 in regulation of anti-inflammatory response to curcumin in 3T3-L1 adipocytes

被引:0
|
作者
Shin, Sunhye [1 ]
Ajuwon, Kolapo M. [2 ,3 ]
机构
[1] Seoul Womens Univ, Div Appl Food Syst, Major Food & Nutr, Seoul 01797, South Korea
[2] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Anim Sci, 270 S Russell St, W Lafayette, IN 47907 USA
关键词
Curcumin; HSP70 heat-shock proteins; NF-kappa B; inflammation; adipocytes; NF-KAPPA-B; FATTY-ACIDS; HSP70; INFLAMMATION; INHIBITION; APOPTOSIS; MODULATION; ACTIVATION; EXPRESSION; PROTECTS;
D O I
10.4162/nrp.2023.17.3.397pISSN1976-1457<bullet>eISSN2005-6168
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
BACKGROUND/OBJECTIVES: Curcumin is a well-known phytochemical with antiinflammatory effects. Heat shock protein (HSP) 70, an intracellular chaperone, inhibits proinflammatory signaling activation. Although curcumin has been shown to induce HSP70 expression in various cell types, whether HSP70 mediates the anti-inflammatory effects of curcumin in mature adipocytes remains unclear. MATERIALS/METHODS: To assess the role of HSP70 in regulating the anti-inflammatory response to curcumin in adipocytes, fully differentiated 3T3-L1 adipocytes were treated with curcumin, lipopolysaccharide (LPS), and/or the HSP70 inhibitor pifithrin-mu (PFT-mu). The expression levels of HSP70 and proinflammatory cytokines were then measured. RESULTS: Curcumin upregulated HSP70 expression at both protein and mRNA levels and attenuated LPS-induced Il6, Ptx3, and Ccl2 mRNA upregulation. PFT-mu tended to exacerbate the LPS-induced upregulation of Il6, Ptx3, Ccl2, and Tnfa mRNA expression. However, on curcumin pretreatment, the tendency of PFT-mu to upregulate LPS-induced proinflammatory cytokine expression decreased or disappeared. CONCLUSION: These results indicate that HSP70 is involved in the regulation of inflammatory responses but may not be crucial for the anti-inflammatory effects of curcumin in 3T3-L1 adipocytes.
引用
收藏
页码:397 / 407
页数:11
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