Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis

被引:5
|
作者
Yuan, Long [1 ]
Zhu, Chuyang [1 ]
Gu, Fang [1 ,2 ]
Zhu, Miaonan [1 ]
Yao, Jiacheng [1 ]
Zhu, Cuipeng [1 ]
Li, Shicheng [1 ]
Wang, Kun [3 ]
Hu, Ping [1 ]
Zhang, Yunzeng [3 ]
Cai, Demin [1 ,2 ]
Liu, Hao-Yu [1 ,2 ]
机构
[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
[2] Yangzhou Univ, Minist Educ China, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China
[3] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
来源
ANIMAL NUTRITION | 2023年 / 15卷
基金
中国国家自然科学基金;
关键词
Lactobacillus johnsonii; Probiotic; Heat shock protein; Diarrhea; Heat stress; Treg/Th17; MICROBIOTA DYSBIOSIS; DENDRITIC CELLS; DIARRHEA; HEALTH;
D O I
10.1016/j.aninu.2023.04.012
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Developing effective strategies to prevent diarrhea and associated-gut disorders in mammals has gained great significance. Owing to the many health benefits provided by the commensal microbiota of the intestinal tract, such as against environmental perturbation, we explored the host phenotype-associated microbes and their probiotic potential. Based on the observations that the chronic heat stress-exposed weaned piglets present as heat stress-susceptible (HS-SUS) or heat stress-resistant (HS-RES) individuals, we confirmed the phenotypic difference between the two on growth performance (P < 0.05), diarrhea index (P < 0.001), intestinal heat shock protein 70 (HSP70) regulation (P < 0.01), and inflammatory responses (P < 0.01). By comparing the gut microbiome using 16S rRNA gene sequencing and KEGG functional analysis, we found that Lactobacillus johnsonii exhibited significantly higher relative abundance in the HS-RES piglets than in the HS-SUS ones (P < 0.05). Further experiments using a mouse model for chemical-induced inflammation and intestinal injury demonstrated that oral administration of a representative L. johnsonii N5 (isolated from the HS-RES piglets) ameliorated the clinical and histological signs of colitis while suppressing intestinal pro-inflammatory cytokines TNF-a and IL-6 production (P < 0.05). We found that N5 treatment enhanced tight junction proteins ZO-1 and occludin and cytoprotective HSP70 levels under physiological condition and restored their mucosal expressions in colitis (P < 0.05). In support of the high production of the anti-inflammatory cytokine IL-10, N5 promoted the intestinal Peyer's patches MHCII thorn and CD103 thorn dendritic cell populations (P < 0.05), increased the regulatory T (Treg) cell numbers (P < 0.05), and decreased the Th17 population and its IL-17a production under physiological condition and during colitis (P < 0.01). Our results shed light on understanding the interaction between commensal Lactobacillus and the host health, and provide L. johnsonii N5 as an alternative to antibiotics for preventing diarrhea and intestinal diseases.(c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
引用
收藏
页码:210 / 224
页数:15
相关论文
共 50 条
  • [41] Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal Cancer via Modulating the Composition of Gut Microbiota and Improving Intestinal Barrier
    Zhu, Huai-Chang
    Jia, Xiao-Kang
    Fan, Yong
    Xu, Shao-Hua
    Li, Xiao-Yan
    Huang, Ming-Qing
    Lan, Meng-Liu
    Xu, Wen
    Wu, Shui-Sheng
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2021, 11
  • [42] Grape seed proanthocyanidin extract ameliorates dextran sulfate sodium-induced colitis through intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokines and gut microbiota modulation
    Sheng, Kangliang
    Zhang, Guanghui
    Sun, Ming
    He, Shiman
    Kong, Xiaowei
    Wang, Jingmin
    Zhu, Fenfang
    Zha, Xiangdong
    Wang, Yongzhong
    FOOD & FUNCTION, 2020, 11 (09) : 7817 - 7829
  • [43] An inulin-type fructan isolated from Serratula chinensis alleviated the dextran sulfate sodium-induced colitis in mice through regulation of intestinal barrier and gut microbiota
    Li, Zhenwei
    Wen, Qiuyi
    Pi, Jiaju
    Zhang, Daidi
    Nie, Jinchun
    Wei, Wenlong
    Li, Wei
    Guo, De-an
    CARBOHYDRATE POLYMERS, 2023, 320
  • [44] A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition
    Saber, Sameh
    Abd El-Fattah, Eslam E.
    Yahya, Galal
    Gobba, Naglaa A.
    Maghmomeh, Abdalkareem Omar
    Khodir, Ahmed E.
    Mourad, Ahmed A. E.
    Saad, Ahmed S.
    Mohammed, Hager G.
    Nouh, Nehal A.
    Shata, Ahmed
    Amin, Noha A.
    Abou El-Rous, Magdy
    Girgis, Samuel
    El-Ahwany, Eman
    Khalaf, Eman M.
    El-Kott, Attalla F.
    El-Baz, Ahmed M.
    PHARMACEUTICALS, 2021, 14 (04)
  • [45] 2-O-β-D-Glucopyranosyl-L-ascorbic Acid, an Ascorbic Acid Derivative Isolated from the Fruits of Lycium Barbarum L., Modulates Gut Microbiota and Palliates Colitis in Dextran Sodium Sulfate-Induced Colitis in Mice
    Huang, Kaiyin
    Dong, Wei
    Liu, Wanyu
    Yan, Yamei
    Wan, Peng
    Peng, Yujia
    Xu, Yujuan
    Zeng, Xiaoxiong
    Cao, Youlong
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (41) : 11408 - 11419
  • [46] Chlorogenic acid supplementation alleviates dextran sulfate sodium (DSS)-induced colitis via inhibiting inflammatory responses and oxidative stress, improving gut barrier integrity and Nrf-2/HO-1 pathway
    Wan, Fan
    Cai, Xueying
    Wang, Mengyu
    Chen, Liang
    Zhong, Ruqing
    Liu, Lei
    Yi, Bao
    Hou, Fujiang
    Zhang, Hongfu
    JOURNAL OF FUNCTIONAL FOODS, 2021, 87
  • [47] Phenolics-Rich Extract from Agarwood Leaf-Tea Alleviate Dextran Sulfate Sodium (DSS)-Induced Ulcerative Colitis Via Modulating Intestinal Barrier Function, Liver Inflammation, and Gut Microbiota
    Fan, Ruyan
    Wei, Wensong
    Wei, Youjing
    Lin, Xue
    Zhou, Shaobo
    Wang, Lu
    MOLECULAR NUTRITION & FOOD RESEARCH, 2024, 68 (24)
  • [48] Therapeutic Efficacy and Underlying Mechanisms of a Mannoglucan from Hirsutella sinensis Mycelium on Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease in Mice: Modulation of the Intestinal Barrier, Oxidative Stress and Gut Microbiota
    Ni, Weihua
    Li, Yu
    Feng, Jingyue
    Liu, Boxuan
    Yuan, Hongyan
    Tai, Guixiang
    Bi, Hongtao
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (23)
  • [49] c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice
    Ren, Qing
    Yang, Bo
    Zhang, Hao
    Ross, R. Paul
    Stanton, Catherine
    Chen, Haiqin
    Chen, Wei
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 68 (12) : 3758 - 3769
  • [50] Orally Administered DHA-Enriched Phospholipids and DHA-Enriched Triglyceride Relieve Oxidative Stress, Improve Intestinal Barrier, Modulate Inflammatory Cytokine and Gut Microbiota, and Meliorate Inflammatory Responses in the Brain in Dextran Sodium Sulfate Induced Colitis in Mice
    Che, Hongxia
    Li, Hongyan
    Song, Lin
    Dong, Xiufang
    Yang, Xihong
    Zhang, Tiantian
    Wang, Yuming
    Xie, Wancui
    MOLECULAR NUTRITION & FOOD RESEARCH, 2021, 65 (15)