FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses

被引：2

作者：

Tiezzi, Camilla ^{[1
]}

Rossi, Marzia ^{[1
]}

Vecchi, Andrea ^{[2
]}

Doselli, Sara ^{[2
]}

Penna, Amalia ^{[2
]}

Fisicaro, Paola ^{[2
]}

Montali, Ilaria ^{[1
]}

Berti, Camilla Ceccatelli ^{[1
]}

Reverberi, Valentina ^{[2
]}

Montali, Anna ^{[1
]}

Missale, Gabriele ^{[1
,2
]}

Ferrari, Carlo ^{[1
,2
]}

Boni, Carolina ^{[2
]}

机构：

[1] Univ Parma, Dept Med & Surg, Parma, Italy

[2] Azienda Ospedaliero Universitaria Parma, Unit Infect Dis & Hepatol, Lab Viral Immunopathol, Parma, Italy

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

关键词：

Cell Biology; Flow Cytometry/Mass Cytometry; Immunology;

D O I：

10.1016/j.xpro.2023.102584

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently de-tecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations.For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).1

引用

页数：13

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