FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses

被引：2
|
作者：
Tiezzi, Camilla [1 ]
Rossi, Marzia [1 ]
Vecchi, Andrea [2 ]
Doselli, Sara [2 ]
Penna, Amalia [2 ]
Fisicaro, Paola [2 ]
Montali, Ilaria [1 ]
Berti, Camilla Ceccatelli [1 ]
Reverberi, Valentina [2 ]
Montali, Anna [1 ]
Missale, Gabriele [1 ,2 ]
Ferrari, Carlo [1 ,2 ]
Boni, Carolina [2 ]
机构：
[1] Univ Parma, Dept Med & Surg, Parma, Italy
[2] Azienda Ospedaliero Universitaria Parma, Unit Infect Dis & Hepatol, Lab Viral Immunopathol, Parma, Italy
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
关键词：
Cell Biology; Flow Cytometry/Mass Cytometry; Immunology;
D O I：
10.1016/j.xpro.2023.102584
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently de-tecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations.For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).1
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页数：13
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