LncRNA H19 Participates in Leukemia Inhibitory Factor Mediated Stemness Promotion in Colorectal Cancer Cells

被引：0
|
作者：
Zhu, Min [1 ,2 ]
Yu, Ruihong [1 ]
Liu, Yirui [1 ]
Geng, Xiaoqing [1 ]
Liu, Qiong [1 ]
Liu, Shuaitong [1 ]
Zhu, Yunhe [3 ]
Li, Gang [3 ]
Guo, Yang [1 ]
Xi, Xueyan [1 ,3 ]
Du, Boyu [1 ,3 ,4 ]
机构：
[1] Hubei Univ Med, Inst Basic Med Sci, 30 Renmin Nanlu, Shiyan 442000, Hubei, Peoples R China
[2] Hubei Univ Med, Sinopharm Dongfeng Gen Hosp, Shiyan, Hubei, Peoples R China
[3] Hubei Univ Med, Renmin Hosp, Shiyan, Hubei, Peoples R China
[4] Hubei Univ Med, Hubei Key Lab Wudang Local Chinese Med Res, Shiyan, Hubei, Peoples R China
来源：
BIOCHEMICAL GENETICS | 2024年 / 62卷 / 05期
关键词：
Colorectal cancer; Leukemia inhibitory factor; H19; AKT; Cancer stem cells; PANCREATIC-CANCER; METASTASIS;
D O I：
10.1007/s10528-023-10627-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Colorectal cancer (CRC) is a common human malignancy and the third leading cause of cancer-related death worldwide. Cancer stem cells (CSCs) were considered to play important roles in the genesis and development of many tumors. In recent years, it has been observed that leukemia inhibitory factor (LIF) might be involved in the regulation of stemness in cancer cells. In this study, we observed that LIF could increase the spheroid formation and stemness marker expression (inculding Nanog and SOX2) in CRC cell lines, such as HCT116 and Caco2 cells. Meanwhile, we also observed that LIF could upregulate LncRNA H19 expression via PI3K/AKT pathway. Knockdown of the expression of LncRNA H19 could decrease the spheroid formation and SOX2 expression in LIF-treated HCT116 and Caco2 cells, and thereby LncRNA H19 knockdown could compensate for the stemness enhancement effects induced by LIF. Our results indicated that LncRNA H19 might participate in the stemness promotion of LIF in CRC cells.
引用
收藏
页码：3695 / 3708
页数：14
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