Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification

被引:5
|
作者
Kok, Sanem Ayaz [1 ,2 ]
Ustun, Selcen [3 ]
Sezgin, Humeyra Taskent [1 ,2 ,3 ]
机构
[1] Izmir Inst Technol, Biotechnol Interdisciplinary Program, TR-35430 Izmir, Turkiye
[2] Teknopk Izmir, New Era Biotechnol, TR-35430 Izmir, Turkiye
[3] Izmir Inst Technol, Bioengn Dept, TR-35430 Izmir, Turkiye
关键词
Isothermal nucleic acid amplification; Pen-side testing; Veterinary diagnostics; Infectious diseases; BOVINE LEUKEMIA-VIRUS; VALLEY FEVER VIRUS; RAPID DETECTION; BLUETONGUE VIRUS; DIARRHEA VIRUS; HERPESVIRUS-1; BHV-1; SENSITIVE DETECTION; VISUAL DETECTION; ANIMAL HEALTH; ORGANIC-DYES;
D O I
10.1007/s12033-023-00674-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Infectious diseases in livestock industry are major problems for animal health, food safety, and the economy. Zoonotic diseases from farm animals are significant threat to human population as well. These are notifiable diseases listed by the World Organization for Animal Health (OIE). Rapid diagnostic methods can help keep infectious diseases under control in herds. Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid amplification method that is studied widely for detection of many infectious diseases in the field. LAMP allows biosensing of target DNA or RNA under isothermal conditions with high specificity in a short period of time. An untrained user can analyze results based on color change or turbidity. Here we review LAMP assays to diagnose OIE notifiable ruminant viral diseases in literature highlighting properties of LAMP method considering what is expected from an efficient, field usable diagnostic test.
引用
收藏
页码:1228 / 1241
页数:14
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