Development and evaluation of in vitro and in vivo antioxidant activity of novel skincare formulation containing phytophospholipid complex

Skin is the outermost layer of the body, continuously exposed to the external environment, harmful UV radiation, chemicals, and air pollutants. Chronic UV radiation exposure induces the production of reactive oxygen species (ROS), and free radicals. Excessive production of free radicals accumulates in the skin and body signifies the oxidative stress. Oxidative stress destroys the antioxidant system of skin, results in number of harmful effects on the skin. Natural phenolic constituents of plant possess strong free radical scavenging and antioxidant potential, prevents the harmful effect of oxidative stress associated with UV radiation and promote the skin health. Low skin permeation of phenolics due to their polarity results in poor solubility, stability, and dermal penetration. Aim of this study was to formulate and evaluate the antioxidant activity of cream formulations containing phytophospholipid complexes of combination of phenolic fractions. Phytophospholipid complex was prepared by solvent evaporation method by incorporating a combination of fraction and soya phospholipid in different molar concentrations (1:1, 1:2, 2:2, 2:1).Various batches of novel skin care cream formulations were prepared by incorporating different concentrations (1%, 2%, 3%, 4%, 5%) of an optimized batch of phytophsopholipid complex. Prepared cream formulations are evaluated for pH, spreadability, viscosity, in vitro antioxidant effect, and in vivo antioxidant effect. The result of pharmaceutical evaluation of all batches of phytosomal cream formulations showed that pharmaceutical parameters are in the normal range. Optimum in vitro and in vivo antioxidant activity are found in novel skin care cream formulation (PCF5) as compared to the conventional cream formulation containing combination of extract.