共 41 条
CgTNF-2 promotes the proliferation of haemocytes by regulating the expressions of CgRunx and cell cycle related genes in the Pacific oyster Crassostrea gigas
被引：5
|作者：
Wu, Wei
[1
,2
,4
]
Sun, Jiejie
[2
,4
]
Dong, Miren
[2
,4
]
Yang, Qian
[2
,4
]
Yang, Wenwen
[2
,4
]
Zhang, Tong
[2
,4
]
Wang, Lingling
[2
,3
,4
,5
]
Song, Linsheng
[2
,3
,4
,5
]
机构：
[1] Liaoning Normal Univ, Sch Life Sci, Dalian 116029, Peoples R China
[2] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Control, Dalian 116023, Peoples R China
[3] Southern Lab Ocean Sci & Engn, Zhuhai 519000, Guangdong, Peoples R China
[4] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
[5] Dalian Ocean Univ, Dalian Key Lab Prevent & Control Aquat Anim Dis, Dalian 116023, Peoples R China
基金：
美国国家科学基金会;
关键词：
Crassostrea gigas;
Tumor necrosis factor;
Granulocytes;
Haemocyte proliferation;
TUMOR-NECROSIS-FACTOR;
FACTOR-ALPHA;
TNF-ALPHA;
MOLECULAR-CLONING;
SUPERFAMILY;
HOMOLOG;
ACTIVATION;
DEPENDENCE;
LOZENGE;
BLOOD;
D O I：
10.1016/j.fsi.2022.108478
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A TNF-alpha family member, CgTNF-2, was previously identified from the oyster Crassostrea gigas to involve in the antibacterial response. In the present study, the role of CgTNF-2 in mediating the proliferation of haemocytes was further explored. The mRNA expression of CgTNF-2 in granulocytes was significantly higher than that in semigranulocytes and agranulocytes, and the percentages of CgTNF-2 antibody labeled cells in agranulocytes, semi-granulocytes and granulocytes were 19.15%, 40.25% and 94.07%, respectively. After the treatment with rCgTNF-2, the percentage of EdU+ cells in haemocytes increased significantly (1.77-fold, p < 0.05) at 6 h compared with that in rGST-treated group, and the mRNA expressions of CgRunx, CgCyclin A, CgCDK2 and CgCDC45 in haemocytes all increased significantly (p < 0.05), which were 1.94-fold, 2.13-fold, 1.97-fold, 1.76fold of that in rGST-treated group, respectively. Meanwhile, the protein abundance of CgRunx and CgCyclin A in the haemocytes of oysters in the rCgTNF-2-treated group increased, and the percentage of PI+ haemocytes in S phase also increased significantly (2.19-fold, p < 0.05) compared with that in rGST-treated group. These results collectively confirmed that CgTNF-2 was highly expressed in granulocytes and involved in the proliferation of haemocytes by regulating the expressions of CgRunx and cell cycle related genes in C. gigas.
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页数：9
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