Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

被引：374

作者：

Welsh, Joshua A. ^{[1
]}

Goberdhan, Deborah C. I. ^{[2
]}

O'Driscoll, Lorraine ^{[3
,4
,5
]}

Buzas, Edit I. ^{[6
,7
,8
]}

Blenkiron, Cherie ^{[9
]}

Bussolati, Benedetta ^{[10
]}

Cai, Houjian ^{[11
]}

Di Vizio, Dolores ^{[12
]}

Driedonks, Tom A. P. ^{[13
]}

Erdbrugger, Uta ^{[14
]}

Falcon-Perez, Juan M. ^{[15
,16
,17
]}

Fu, Qing-Ling ^{[18
,19
]}

Hill, Andrew F. ^{[20
]}

Lenassi, Metka ^{[21
]}

Lim, Sai Kiang ^{[22
,23
,24
]}

Mahoney, My G. ^{[25
]}

Mohanty, Sujata ^{[26
]}

Moller, Andreas ^{[27
,28
]}

Nieuwland, Rienk ^{[29
,30
]}

Ochiya, Takahiro ^{[31
]}

Sahoo, Susmita ^{[32
]}

Torrecilhas, Ana C. ^{[33
]}

Zheng, Lei ^{[34
]}

Zijlstra, Andries ^{[35
,36
]}

Abuelreich, Sarah ^{[37
]}

Bagabas, Reem ^{[37
]}

Bergese, Paolo ^{[38
,39
,40
]}

Bridges, Esther M. ^{[41
]}

Brucale, Marco ^{[42
,43
]}

Burger, Dylan ^{[44
,45
,46
]}

Carney, Randy P. ^{[47
]}

Cocucci, Emanuele ^{[48
,49
]}

Colombo, Federico ^{[48
]}

Crescitelli, Rossella ^{[50
,51
]}

Hanser, Edveena ^{[52
,53
]}

Harris, Adrian L. ^{[54
]}

Haughey, Norman J. ^{[55
,56
]}

Hendrix, An ^{[57
,58
]}

Ivanov, Alexander R. ^{[59
]}

Jovanovic-Talisman, Tijana ^{[60
]}

Kruh-Garcia, Nicole A. ^{[61
]}

Faustino, Vroniqa Ku'ulei-Lyn ^{[37
]}

Kyburz, Diego ^{[53
,62
]}

Lasser, Cecilia

Lennon, Kathleen M. ^{[37
]}

Lotvall, Jan ^{[63
,64
]}

Maddox, Adam L. ^{[37
]}

Martens-Uzunova, Elena S. ^{[65
]}

Mizenko, Rachel R. ^{[47
]}

Newman, Lauren A. ^{[66
]}

机构：

[1] NCI, Translat Nanobiol Sect, Lab Pathol, NIH, Bethesda, MD 20892 USA

[2] Univ Oxford, John Radcliffe Hosp, Womens Ctr, Nuffield Dept Womens & Reprod Hlth, Oxford, England

[3] Trinity Coll Dublin, Sch Pharm & Pharmaceut Sci, Dublin, Ireland

[4] Trinity Coll Dublin, Trinity Biomed Sci Inst, Dublin, Ireland

[5] Trinity Coll Dublin, Trinity St Jamess Canc Inst, Dublin, Ireland

[6] Semmelweis Univ, Dept Genet Cell and Immunobiol, Budapest, Hungary

[7] Semmelweis Univ, HCEMM SU Extracellular Vesicle Res Grp, Budapest, Hungary

[8] Semmelweis Univ, HUN REN SU Translat Extracellular Vesicle Res Grp, Budapest, Hungary

[9] Univ Auckland, Fac Med & Hlth Sci, Auckland, New Zealand

[10] Univ Turin, Dept Mol Biotechnol & Hlth Sci, Turin, Italy

[11] Univ Georgia, Athens, GA USA

[12] Cedars Sinai Med Ctr, Dept Surg, Div Canc Biol & Therapeut, Los Angeles, CA 90048 USA

[13] Univ Med Ctr Utrecht, Dept CDL Res, Utrecht, Netherlands

[14] Univ Virginia Hlth Syst, Charlottesville, VA USA

[15] Basque Res & Technol Alliance, Ctr Cooperat Res Biosci, Exosomes Lab, Derio, Spain

[16] Basque Res & Technol Alliance, Ctr Cooperat Res Biosci, Metabol Platform, Derio, Spain

[17] Basque Fdn Sci, Ikerbasque, Bilbao, Spain

[18] Sun Yat Sen Univ, Otorhinolaryngol Hosp, Affiliated Hosp 1, Guangzhou, Peoples R China

[19] Sun Yat Sen Univ, Affiliated Hosp 1, Extracellular Vesicle Res & Clin Translat Ctr, Guangzhou 510080, Peoples R China

[20] Victoria Univ, Inst Hlth & Sport, Melbourne, Vic, Australia

[21] Univ Ljubljana, Fac Med, Ljubljana, Slovenia

[22] ASTAR, Inst Mol & Cell Biol IMCB, Singapore, Singapore

[23] Paracrine Therapeut Pte Ltd, Singapore, Singapore

[24] Natl Univ Singapore, YLL Sch Med, Dept Surg, Singapore, Singapore

[25] Thomas Jefferson Univ, Philadelphia, PA USA

[26] All India Inst Med Sci, Stem Cell Facil, New Delhi, India

[27] Chinese Univ Hong Kong, Hong Kong, Peoples R China

[28] QIMR Berghofer Med Res Inst, Brisbane, Australia

[29] Univ Amsterdam, Locat AMC, Amsterdam Univ Med Ctr, Med Ctr,Lab Expt Clin Chem, Amsterdam, Netherlands

[30] Univ Amsterdam, Amsterdam Univ, Amsterdam Vesicle Ctr, Med Ctr,Locat AMC, Amsterdam, Netherlands

[31] Tokyo Med Univ, Tokyo, Japan

[32] Icahn Sch Med Mt Sinai, New York, NY 10029 USA

[33] Univ Fed Sao Paulo UNIFESP Campus Diadema, Dept Ciencias Farmaceut, Lab Imunol Celular & Bioquim Fungos & Protozoario, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, Brazil

[34] Southern Med Univ, Nanfang Hosp, Dept Lab Med, Guangzhou, Peoples R China

[35] Vanderbilt Univ, Med Ctr, Dept Pathol, Nashville, TN 37232 USA

[36] Genentech Inc, San Francisco, CA USA

[37] City Hope Comprehens Canc Ctr, Beckman Res Inst, Dept Mol Med, Duarte, CA 91010 USA

[38] Univ Brescia, Dept Mol & Translat Med, Brescia, Italy

[39] Ctr Colloid & Surface Sci CSGI, Florence, Italy

[40] Natl Ctr Gene Therapy & Drugs Based RNA Technol C, Padua, Italy

[41] Univ Oxford, Weatherall Inst Mol Med, Oxford, England

[42] CNR, Ist Studio Mat Nanostrutturati, Bologna, Italy

[43] Consorzio Interuniv Sviluppo Sistemi Grande Inter, Florence, Italy

[44] Ottawa Hopsital Res Inst, Kidney Res Ctr, Ottawa, ON, Canada

[45] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada

[46] Univ Ottawa, Sch Pharmaceut Sci, Ottawa, ON, Canada

[47] Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA

[48] Ohio State Univ, Coll Pharm, Div Pharmaceut & Pharmacol, 500 W 12Th Ave, Columbus, OH 43210 USA

[49] Ohio State Univ, Comprehens Canc Ctr, Columbus, OH 43210 USA

[50] Univ Gothenburg, Sahlgrenska Acad, Sahlgrenska Ctr Canc Res, Dept Surg,Inst Clin Sci, Gothenburg, Sweden

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2024年 / 13卷 / 02期

关键词：

ectosomes; exosomes; extracellular vesicles; extracellular particles; guidelines; microparticles; microvesicles; minimal information requirements; MISEV; reproducibility; rigor; standardisation; FLUID PROTEIN-CONCENTRATION; HUMAN CEREBROSPINAL-FLUID; DOUBLE-STRANDED DNA; MASS-SPECTROMETRY; FLOW-CYTOMETRY; IN-VITRO; SYSTEM SUITABILITY; RAMAN-SPECTROSCOPY; PROTEOMIC ANALYSIS; MEMBRANE-VESICLES;

D O I：

10.1002/jev2.12404

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

引用

页数：84

共 50 条

[1] Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches (vol 13, e12404, 2023)
Welsh, J. A.
Goberdhan, D. C., I
O'Driscoll, L.
Buzas, E., I
Blenkiron, C.
Bussolati, B.
Cai, H.
Di Vizio, D.
Driedonks, T. A. P.
Erdbrugger, U.
Falcon-Perez, J. M.
Fu, Q. L.
Hill, A. F.
Lenassi, M.
Lim, S. K.
Mahoney, M. G.
Mohanty, S.
Moller, A.
Nieuwland, R.
Ochiya, T.
Sahoo, S.
Torrecilhas, A. C.
Zheng, L.
Zijlstra, A.
Abuelreich, S.
Bagabas, R.
Bergese, P.
Bridges, E. M.
Brucale, M.
Burger, D.
Carney, R. P.
Cocucci, E.
Crescitelli, R.
Hanser, E.
Harris, A. L.
Haughey, N. J.
Hendrix, A.
Ivanov, A. R.
Jovanovic-Talisman, T.
Kruh-Garcia, N. A.
Faustino, V. Kuulei-Lyn
Kyburz, D.
Lasser, C.
Lennon, K. M.
Lotvall, J.
Maddox, A. L.
Martens-Uzunova, E. S.
Mizenko, R. R.
Newman, L. A.
Ridolfi, A.
[J]. JOURNAL OF EXTRACELLULAR VESICLES, 2024, 13 (05)
[2] Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches (vol 13, e12404, 2024)
Welsh, J. A.
Goberdhan, D. C., I
O'Driscoll, L.
Buzas, E., I
Blenkiron, C.
Bussolati, B.
Cai, H.
Di Vizio, D.
Driedonks, T. A. P.
Erdbrugger, U.
Falco-Perez, J. M.
Fu, Q. L.
Hill, A. F.
Lenassi, M.
Lim, S. K.
Mahoney, M. G.
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[J]. JOURNAL OF EXTRACELLULAR VESICLES, 2024, 13 (05)
[3] MISEV2023 provides an updated and key reference for researchers studying the basic biology and applications of extracellular vesicles
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[J]. STEM CELLS TRANSLATIONAL MEDICINE, 2024, 13 (09) : 848 - 850
[4] Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles
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[J]. JOURNAL OF EXTRACELLULAR VESICLES, 2021, 10 (14)
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[J]. JOURNAL OF EXTRACELLULAR VESICLES, 2018, 7 (01)
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[J]. SEMINARS IN CANCER BIOLOGY, 2024, 101 : 12 - 24
[8] Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
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