Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

被引：2168

作者：

Lotvall, Jan ^{[1
]}

Hill, Andrew F. ^{[2
]}

Hochberg, Fred ^{[3
]}

Buzas, Edit I. ^{[4
]}

Di Vizio, Dolores ^{[5
]}

Gardiner, Christopher ^{[6
]}

Gho, Yong Song ^{[7
]}

Kurochkin, Igor V. ^{[8
]}

Mathivanan, Suresh ^{[9
]}

Quesenberry, Peter ^{[10
]}

Sahoo, Susmita ^{[11
]}

Tahara, Hidetoshi ^{[12
]}

Wauben, Marca H. ^{[13
]}

Witwer, Kenneth W. ^{[14
]}

Thery, Clotilde ^{[15
]}

机构：

[1] Univ Gothenburg, Krefting Res Ctr, Gothenburg, Sweden

[2] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic, Australia

[3] Univ Calif San Diego, Dept Neurosurg, San Diego, CA 92103 USA

[4] Semmelweis Univ, Dept Genet Cell & Immunobiol, Budapest, Hungary

[5] Cedars Sinai Med Ctr, Los Angeles, CA 90048 USA

[6] Univ Oxford, Nuffield Dept Obstet & Gynaecol, Oxford, England

[7] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea

[8] Bioinformat Inst, Singapore, Singapore

[9] La Trobe Univ, Dept Biochem, Melbourne, Vic, Australia

[10] Brown Univ, Warren Alpert Med Sch, Providence, RI 02912 USA

[11] Icahn Sch Med Mt Sinai, Cardiovasc Res Ctr, New York, NY 10029 USA

[12] Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Cellular & Mol Biol, Hiroshima, Japan

[13] Univ Utrecht, Fac Vet Med, Dept Biochem & Cell Biol, Utrecht, Netherlands

[14] Johns Hopkins Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD USA

[15] Inst Curie, INSERM U932, F-75005 Paris, France

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2014年 / 3卷 / 01期

关键词：

extracellular vesicles; microvesicles; microparticles; exosomes; ectosomes; extracellular RNA;

D O I：

10.3402/jev.v3.26913

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

引用

页数：6

共 50 条

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