Influence of substrate/inoculum ratio, inoculum source and ammonia inhibition on anaerobic digestion of poultry waste

Poultry wastes are rich in organic matter, allowing their use as substrates for biogas production by anaerobic digestion (AD). The major difficulty in the anaerobic digestion of this protein-rich waste is ammonia inhibition. Different results of biochemical methane potential (BMP) were obtained after the mesophilic anaerobic digestion of different avian waste in batch mode. It was shown that using two different inoculum (Liger and Saint-Brieuc) sources and different substrate-to-inoculum (S/I) ratios does not have a significant effect on the biochemical methane potential of organic laying hen droppings (OLHD); an average of 0.272 Nm(3) CH4 center dot kg(-1)center dot VS was obtained with both inocula. Otherwise, it affects the hydrolysis constant K-H, and it decreases when the substrate-to-inoculum ratio increases. Furthermore, Liger is the most suitable inoculum for our substrate because it shows stability during the process even with different organic loads. Comparing the biochemical methane potential of multiple avian wastes such as organic laying hen droppings and different slaughterhouse waste highlights the importance of slaughterhouse waste in the anaerobic digestion process because of the high methane yield observed especially with the viscera (0.779 Nm(3) CH4 center dot kg(-1) VS, SD = 0.027 Nm(3) CH4 center dot kg(-1) VS). Moreover, methane production was affected by increasing the ammonia concentrations; when [N-NH3] > 9.8 g center dot N-NH3 center dot L-1, the biochemical methane potential decreases and the lag phase increases (lambda > 30 days); a total inhibition of the process was observed when ammonia concentration is above 21.8 g center dot L-1.