Study of the effects of various cryopreservation methods on viability of human gastrointestinal tumor xenografts in in vivo models

The efficiency of 3 protocols of cryopreservation of tumor tissue obtained from patients with malignant formations of the gastrointestinal tract: esophagus, stomach, large intestine was evaluated. The experiment was performed on 90 Balb/c Nude mice. After 90 days from the moment of freezing of tumor nodes, the samples were thawed and implanted in animals, the number of surviving implants was noted and the beginning of growth of tumor nodes in animals was recorded. For cryopreservation of xenografts of human esophageal and colon cancer, it is possible to use protocols 2 and 3 tested by us, where the key feature is the slow freezing of samples. For the tumor material of the stomach, it is necessary to develop other methods of preservation, due to the low efficiency of the methods considered in the work.