Preparation of C-Terminal Epitope Imprinted Particles for Recognition of Cytochrome c Assisted with ?-Cyclodextrin by Host-Guest Interaction

A kind of epitope surface imprinted particles was synthesized to selectively recognize cytochrome c (Cyt c) by a novel strategy assisted with ?-cyclodextrin (?-CD) by host-guest interaction. C-terminal epitope nonapeptide of Cyt c was chosen as the template. ?-CD was immobilized on the surface of the silica as an encapsulated molecule to capture the template and improve the corresponding spatial orientation by the host-guest interaction for the peptide and target protein recognition. After ?-CD and epitope modified, the surface imprinted polymer assisted with the host-guest interaction was synthesized via monomers and cross-linkers by radical polymerization strategy. After the peptide removal, the epitope surface imprinted particles were obtained. The performance of Cyt c recognition in bovine serum sample by the imprinted polymers was calculated by HPLC. The modified imprinted polymers prepared achieved the best binding capability of 2.89 mg g(-1) AE-9 with the IF = 4.07 and 37.58 mg g(-1) Cyt c with the IF = 3.38, and it also realized an efficiency and selectivity protein recognition. Additionally, the imprinted particles assisting by ?-CD have good reusability with 89.89% of the original after five sorption-elution cycles and a better recognized ability (IF = 3.38) than those without ?-CD assisting (IF = 1.44). These results indicated that this epitope imprinted method assisted with the host-guest recognition interaction by ?-CD exhibited well specific recognition abilities towards the target protein Cyt c and potential application for Cyt c recognition in biological sample.