Protocol for near-infrared optogenetics manipulation of neurons and motor behavior in C. elegans using emissive upconversion nanoparticles

被引：0
|
作者：
Wang, Ruipeng [1 ]
Guo, Jingxuan [1 ,2 ]
Yao, Hanlu [1 ]
Luo, Xuekai [1 ]
Deng, Yixiang [1 ]
Tian, Yuhang [1 ]
Zhang, Yan [1 ,2 ]
Gao, Shangbang [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China
[2] Huazhong Univ Sci & Technol, Natl Engn Res Ctr Nanomed, Wuhan 430074, Peoples R China
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 01期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
Biophysics; Biotechnology and bioengineering; Neuroscience;
D O I：
10.1016/j.xpro.2024.102858
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In deep tissue, optogenetics faces limitations with visible light. Here, we present a protocol for near -infrared (NIR) optogenetics manipulation of neurons and motor behavior in Caenorhabditis elegans using emissive upconversion nanoparticles (UCNPs). We describe steps for synthesizing and modifying UCNPs. We then detail procedures for regulating neurons using these UCNPs in the model organism C. elegans. Using NIR light allows for superior tissue penetration to manipulate neuronal activities and locomotion behavior. For complete details on the use and execution of this protocol, please refer to Guo et al.,1 Ao et al.,2 and Zhang et al.3
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页数：24
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