Characterization of polymorphisms in the follicle-stimulating hormone receptor and insulin-like growth factor-1 genes and their association with fertility traits in Jawa-Brebes cows

被引:1
|
作者
Hartanto, Slamet [1 ,2 ]
Budiyanto, Agung [1 ]
Widayanti, Rini [3 ]
Setyawan, Erif Maha Nugraha [1 ]
Prasetya, Imawan Daru [4 ]
机构
[1] Gadjah Mada Univ, Fac Vet Med, Dept Reprod Obstet & Gynecol, Yogyakarta, Indonesia
[2] Natl Res & Innovat Agcy BRIN, Jakarta, Indonesia
[3] Univ Gadjah Mada, Fac Vet Med, Dept Biochem & Mol Biol, Yogyakarta, Indonesia
[4] Minist Agr, Directorate Gen Livestock & Anim Hlth, Jakarta, Indonesia
关键词
fecundity; genetic marker; Indonesian cow; restriction fragment length polymorphism-polymerase chain reaction; LITTER SIZE; FSHR GENE; REGION; CATTLE; EXPRESSION; IMPACT; DNA;
D O I
10.14202/vetworld.2023.711-716
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Background and Aim: The availability of fertility markers is crucial for maintaining, protecting, and improving the genetics of Jawa-Brebes (Jabres) cows. Follicle-stimulating hormone receptor (FSHR) and insulin-like growth factor-1 (IGF-1) play critical roles in female reproductive physiology. The single-nucleotide polymorphisms (SNPs) FSHR G-278A and IGF-1 C-512T correlate with cows' fertility traits. This study aimed to identify these SNPs and their potential associations with fertility parameters in Jabres cows.Materials and Methods: Samples were collected from 45 heads of multiparous Jabres cows aged 3-10 years with body condition scores of 2.5-5.0 on a 5-point scale in Brebes Regency, Java, Indonesia. These cows were assigned to fertile (n = 16) and infertile groups (n = 29). Polymerase chain reaction (PCR) was carried out for DNA amplification of FSHR G-278A and IGF-1 C-512T fragments. Restriction fragment length polymorphism-PCR with the restriction enzymes FaqI for the product of FSHR G-278A and SnaBI for the product of IGF-1 C-512T was used to identify SNPs.Results: The FaqI enzyme cut the 211 bp DNA fragment of FSHR G-278A in all samples into two bands of 128 bp and 83 bp (GG genotype). Meanwhile, the genotyping of amplicon products of IGF-1 C-512T generated a single 249 bp fragment (CC genotype) in both groups.Conclusion: The results showed that the FSHR G-278A/FaqI and IGF-1 C-512T/SnaBI loci were monomorphic in Jabres cows. Thus, neither FSHR G-278A/FaqI nor IGF-1 C-512T/SnaBI is a possible genetic marker for fertility in Jabres cows.
引用
收藏
页码:711 / 716
页数:6
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