High-resolution imaging of the whole eye with photoacoustic microscopy

Observation and characterization of any changes in anatom-ical structures of ocular components remain as a conven-tional technique for diagnosis, staging, therapeutic treat-ments, and post-treatment monitoring of any ophthalmic disorders. The existing technologies fail to provide imaging of all of the various components of the eye simultaneously at one scanning time, i.e., one can recover vital patho-physiological information (structure and bio-molecular con-tent) of the different ocular tissue sections only one after another. This article addresses the longstanding technologi-cal challenge by use of an emerging imaging modality [pho-toacoustic imaging (PAI)] in which we integrated a synthetic aperture reconstruction technique (SAFT). Experimental results-with experiments being conducted in excised tis-sues (goat eye)-demonstrated that we can simultaneously image the entire structure of the eye (& SIM;2.5 cm) depicting clearly the distinctive ocular structures (cornea, aqueous humor, iris, pupil, eye lens, vitreous humor, and retina). This study uniquely opens an avenue for promising ophthalmic (clinical) applications of high clinical impact.& COPY; 2023 Optica Publishing Group