Assessing the Biosynthetic Inventory of the Biocontrol Fungus Trichoderma afroharzianum T22

被引：3
|
作者：
Han, Wenyu [1 ]
Wu, Zhongshou [2 ,3 ]
Zhong, Zhenhui [2 ,3 ]
Williams, Jason [1 ]
Jacobsen, Steven E. [2 ,3 ,4 ,5 ]
Sun, Zuodong [6 ]
Tang, Yi [1 ,6 ]
机构：
[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell R, Los Angeles, CA 90095 USA
[5] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA
[6] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 30期
关键词：
biocontrol and biofertilizer fungi; secondary metabolite; natural product; biosynthesis; transcriptomics; OSMAC; GENE-CLUSTER; SECONDARY METABOLITES; NATURAL-PRODUCTS; HARZIANUM; ACID; IDENTIFICATION; DISCOVERY; COMPLEX; LIPOPEPTIDES; VERTICILLIN;
D O I：
10.1021/acs.jafc.3c03240
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Natural products biosynthesized from biocontrol fungiin the rhizospherecan have both beneficial and deleterious effects on plants. Herein,we performed a comprehensive analysis of natural product biosyntheticgene clusters (BGCs) from the widely used biocontrol fungus Trichoderma afroharzianum T22 (ThT22). This fungusencodes at least 64 BGCs, yet only seven compounds and four BGCs werepreviously characterized or mined. We correlated 21 BGCs of ThT22with known primary and secondary metabolites through homologous BGCcomparison and characterized one unknown BGC involved in the biosynthesisof eujavanicol A using heterologous expression. In addition, we performeduntargeted transcriptomics and metabolic analysis to demonstrate theactivation of silent ThT22 BGCs via the "one strain many compound"(OSMAC) approach. Collectively, our analysis showcases the biosyntheticcapacity of ThT22 and paves the way for fully exploring the rolesof natural products of ThT22.
引用
收藏
页码：11502 / 11519
页数：18
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