E. coli catheter-associated urinary tract infections are associated with distinctive virulence and biofilm gene determinants

被引:10
|
作者
Zou, Zongsen [1 ,2 ,10 ]
Potter, Robert F. [3 ,4 ,5 ]
McCoy, William H. [1 ]
Wildenthal, John A. [1 ,2 ]
Katumba, George L. [1 ,2 ]
Mucha, Peter J. [6 ]
Dantas, Gautam [4 ,5 ,7 ,8 ]
Henderson, Jeffrey P. [1 ,2 ,9 ]
机构
[1] Washington Univ, Ctr Womens Infect Dis Res, Sch Med, St Louis, MO 63110 USA
[2] Washington Univ, Dept Internal Med, Div Infect Dis, Sch Med, St Louis, MO 63110 USA
[3] Washington Univ, Edison Family Ctr Genome Sci & Syst Biol, Sch Med, St Louis, MO 63110 USA
[4] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA
[5] Washington Univ, Dept Internal Med, Div Dermatol, Sch Med, St Louis, MO 63110 USA
[6] Dartmouth Coll, Dept Math, Hanover, NH USA
[7] Washington Univ, Dept Mol Microbiol, Sch Med, St Louis, MO 63110 USA
[8] Washington Univ, Dept Biomed Engn, St Louis, MO 63110 USA
[9] Washington Univ, Sch Med, Box 8051,660 S Euclid Ave, St Louis, MO 63110 USA
[10] Washington Univ, Ctr Womens Infect Dis Res, Dept Mol Microbiol, Sch Med, St Louis, MO 63110 USA
关键词
UROPATHOGENIC ESCHERICHIA-COLI; ASYMPTOMATIC BACTERIURIA; OUTER-MEMBRANE; ANTIBIOTIC-RESISTANCE; DISEASES SOCIETY; IRON; INTERFERENCE; PREVENTION; MANAGEMENT; ADHERENCE;
D O I
10.1172/jci.insight.161461
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Urinary catheterization facilitates urinary tract colonization by E. coli and increases infection risk. Here, we aimed to identify strain-specific characteristics associated with the transition from colonization to infection in catheterized patients. In a single-site study population, we compared E. coli isolates from patients with catheter-associated asymptomatic bacteriuria (CAASB) to those with catheter-associated urinary tract infection (CAUTI). CAUTI isolates were dominated by a phylotype B2 subclade containing the multidrug-resistant ST131 lineage relative to CAASB isolates, which were phylogenetically more diverse. A distinctive combination of virulence-associated genes was present in the CAUTI-associated B2 subclade. Catheter-associated biofilm formation was widespread among isolates and did not distinguish CAUTI from CAASB strains. Preincubation with CAASB strains could inhibit catheter colonization by multiple ST131 CAUTI isolates. Comparative genomic analysis identified a group of variable genes associated with high catheter biofilm formation present in both CAUTI and CAASB strains. Among these, ferric citrate transport (Fec) system genes were experimentally associated with enhanced catheter biofilm formation using reporter and fecA deletion strains. These results are consistent with a variable role for catheter biofilm formation in promoting CAUTI by ST131-like strains or resisting CAUTI by lower-risk strains that engage in niche exclusion.
引用
收藏
页数:19
相关论文
共 50 条
  • [31] Reducing catheter-associated urinary tract infections in the ICU
    Sampathkumar, Priya
    CURRENT OPINION IN CRITICAL CARE, 2017, 23 (05) : 372 - 377
  • [32] Catheter-Associated Urinary Tract Infection and Obstinate Biofilm Producers
    Maharjan, Govinda
    Khadka, Priyatam
    Shilpakar, Gomik Siddhi
    Chapagain, Ganesh
    Dhungana, Guna Raj
    CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY, 2018, 2018
  • [33] Urinary Catheter-Associated Infections
    Scruggs-Wodkowski, Elizabeth
    Kidder, Ian
    Meddings, Jennifer
    Patel, Payal K.
    INFECTIOUS DISEASE CLINICS OF NORTH AMERICA, 2024, 38 (04) : 713 - 729
  • [34] Urinary Catheter-Associated Infections
    Shuman, Emily K.
    Chenoweth, Carol E.
    INFECTIOUS DISEASE CLINICS OF NORTH AMERICA, 2018, 32 (04) : 885 - +
  • [35] Urinary Catheter-Associated Infections
    Nicolle, Lindsay E.
    INFECTIOUS DISEASE CLINICS OF NORTH AMERICA, 2012, 26 (01) : 13 - +
  • [36] Review of Catheter-Associated Urinary Tract Infections and In Vitro Urinary Tract Models
    Cortese, Yvonne J.
    Wagner, Victoria E.
    Tierney, Morgan
    Devine, Declan
    Fogarty, Andrew
    JOURNAL OF HEALTHCARE ENGINEERING, 2018, 2018
  • [37] Catheter-associated Urinary Tract Infections in Children: Room for Improvement Commentary on: Reducing Catheter-associated Urinary Tract Infections: A Quality-improvement Initiative
    Shakeri, Aria
    Elterman, Dean S.
    UROLOGY, 2015, 85 (05) : 967 - 968
  • [38] Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis
    Jacobsen, S. M.
    Stickler, D. J.
    Mobley, H. L. T.
    Shirtliff, M. E.
    CLINICAL MICROBIOLOGY REVIEWS, 2008, 21 (01) : 26 - +
  • [39] Catheter-associated urinary tract infections: impact of catheter materials on their management
    Kumon, H
    Hashimoto, H
    Nishimura, M
    Monden, K
    Ono, N
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2001, 17 (04) : 311 - 316
  • [40] Reducing Escherichia coli bacteraemia associated with catheter-associated urinary tract infections in the secondary care setting
    Bradley, C. W.
    Flavell, H.
    Raybould, L.
    McCoy, H.
    Dempster, L.
    Holden, E.
    Garvey, M. I.
    JOURNAL OF HOSPITAL INFECTION, 2018, 98 (03) : 236 - 237