Dependence of the solute retention on the column pressure in reversed-phase HPLC

被引：0
|
作者：
Deineka, Viktor I. [1 ]
Chulkov, Andrey N. [2 ]
Blinova, Irina P. [1 ]
机构：
[1] Belgorod State Natl Res Univ, Belgorod 308015, Russia
[2] FGBU Ctr Assessment Grain Qual, Belgorog Branch, Belgorod 308027, Russia
来源：
MENDELEEV COMMUNICATIONS | 2023年 / 33卷 / 03期
关键词：
reversed-phase HPLC; retention; anthocyanins; pressure dependence; mechanism of retention; ODS stationary phase structure; CHROMATOGRAPHY; PARAMETERS;
D O I：
10.1016/j.mencom.2023.04.044
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In the course of reversed-phase HPLC analysis, increase in pressure at the column inlet can affect the retention of sorbates only when they penetrate into the grafted phase. Due to conformational changes in the alkyl groups of the loose grafted layer, the solvation of sorbates by these alkyl groups would increase, leading to a growth of the retention factors. In the case of the surface sorption of solutes, the effect is not observed.
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页码：436 / 437
页数：2
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